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X Wang, B Song, X Qiu (2009)
Development of EST-SSRs in scallop (Patinopecten yessoensis) from sequence databaseConserv Genet, 10
SY Lee, SH Kim (2003)
Genetic variation and discrimination of Korean ark shell Scapharca species (Bivalvia, Arcoida) based on mitochondrial COI gene sequences and PCR-RFLPKorean J Genet, 25
QZ Wang, M Zhang, WM Teng (2015)
A review: research progress of biology in ark shell Scapharca subcrenata insight from aquacultureJ Dalian Ocean Univ, 30
MR Garvin, K Saitoh, AJ Gharrett (2010)
Application of single nucleotide polymorphisms to non-model species: a technical reviewMol Ecol Res, 10
BS Weir, CC Cockerham (1984)
Estimating F-statistics for the analysis of population structureEvol Int J Org Evol, 38
SY Chen, HJ Ji, SX Xu (2009)
Polymorphic microsatellite DNA markers for the ark shell Scapharca subcrenata (bivalve: Arcidae)Conserv Genet, 10
Y Jin, Q Li, L Kong (2014)
High-resolution melting (HRM) analysis: a highly sensitive alternative for the identification of commercially important Crassostrea oystersJ Mollus Stud, 81
K Belkhir, P Borsa, L Chikhi, N Raufaste, F Bonhomme (2004)
GENETIX 4.05.2, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions, CNRS UMR 5000
SY Kim, JY Kim, JM Yoon (2009)
Genetic variability between Ark Shell (Scapharca subcrenata, Lischke) populations from Daecheon and WonsanKorean J Malacol, 25
X Sun, G Shin, D Hedgecock (2015)
Inheritance of high-resolution melting profiles in assays targeting single nucleotide polymorphisms in protein-coding sequences of the pacific oyster crassostrea gigas: implications for parentage assignment of experimental and commercial broodstocksAquaculture, 437
YW Feng, Q Li, LF Kong (2009)
Isolation and characterization of 14 polymorphic microsatellite loci in the ark shell Scapharca subcrenata, (Bivalvia: Arcidae)Conserv Genet, 10
Q Li, C Park, T Kobayashi, A Kijima (2003)
Inheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannaiMar Biotechnol, 5
M Raymond, F Rousset (1995)
GENEPOP (version 1.2): population genetics software for exact tests and ecumenicismJ Hered, 86
The ark shell Scapharca subcrenata, an important fishery resource in Asian countries, has been suffering from severe population decline in recent years. To facilitate genetic conservation studies, novel single nucleotide polymorphisms (SNPs) markers were developed using next generation sequencing for this species, which yielded a total of 781,860 putative SNPs. To validate these markers, 96 SNP genotyping assays were designed to characterize polymorphisms using high resolution melting (HRM) analysis in 32 individuals from a wild population. As a result, 37 SNPs showed polymorphisms with MAF ranging from 0.0313 to 0.4844. The observed heterozygosity ranged from 0 to 0.8333, while the expected heterozygosity varied from 0.0605 to 0.4995. After the Bonferroni correction, 5 of the 37 loci showed significant departure from Hardy–Weinberg equilibrium. These novel SNP markers will be useful for future investigation of population structure and conservation genetics of the ark shell S. subcrenata.
Conservation Genetics Resources – Springer Journals
Published: Jan 10, 2017
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