Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations

Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations Because the mouse has become the pre-eminent model system for functional genomics and analysis of complex-systems/pathways in mammals, there has been an escalation of interest in the generation and analysis of mouse mutations to use as tools in these analyses. I argue here for a parallel investment in continuing the development of appropriately marked chromosomal rearrangements to use as genetic reagents in mutation recovery, analysis, and maintenance crosses. Specifically, visibly marked interstitial chromosomal deletions can be valuable for regional mutagenesis screens for recessives based on hemizygosity, and they can also be used to simplify genetic fine-mapping as a prelude to gene identification based on positional cloning/candidacy strategies. Dominantly marked chromosomal inversions that also manifest some kind of recessive phenotype can be exploited in more extensive regional mutagenesis screens based on homozygosity, and are invaluable for simplified, low-cost and error-reduced mutant-stock maintenance. Also discussed are several issues concerning genetic background, particularly from the point of view of genetic-reagent resource development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations

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Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003350010095
Publisher site
See Article on Publisher Site

Abstract

Because the mouse has become the pre-eminent model system for functional genomics and analysis of complex-systems/pathways in mammals, there has been an escalation of interest in the generation and analysis of mouse mutations to use as tools in these analyses. I argue here for a parallel investment in continuing the development of appropriately marked chromosomal rearrangements to use as genetic reagents in mutation recovery, analysis, and maintenance crosses. Specifically, visibly marked interstitial chromosomal deletions can be valuable for regional mutagenesis screens for recessives based on hemizygosity, and they can also be used to simplify genetic fine-mapping as a prelude to gene identification based on positional cloning/candidacy strategies. Dominantly marked chromosomal inversions that also manifest some kind of recessive phenotype can be exploited in more extensive regional mutagenesis screens based on homozygosity, and are invaluable for simplified, low-cost and error-reduced mutant-stock maintenance. Also discussed are several issues concerning genetic background, particularly from the point of view of genetic-reagent resource development.

Journal

Mammalian GenomeSpringer Journals

Published: Feb 25, 2014

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