ISSN 1070-4272, Russian Journal of Applied Chemistry, 2015, Vol. 88, No. 1, pp. 72−81. © Pleiades Publishing, Ltd., 2015.
Original Russian Text © I.S. Yaroshenko, D.O. Kirsanov, P. Wang, D. Ha, H. Wan, J. He, Yu.G. Vlasova, A.V. Legin, 2015, published in Zhurnal Prikladnoi Khimii,
2015, Vol. 88, No. 1, pp. 78−87.
OF SYSTEMS AND PROCESSES
Determination of the Toxicity of Herb Preparations
of the Traditional Chinese Medicine with a Multisensor System
I. S. Yaroshenko
, D. O. Kirsanov
, P. Wang
, D. Ha
, H. Wan
, J. He
Yu. G. Vlasov
, and A. V. Legin
Institute of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Petrodvorets, St. Petersburg, 198504 Russia
Laboratory of Artiﬁ cial Sensor Systems, ITMO University, Kronverkskii pr. 49, St. Petersburg, 197101 Russia
Chair of Biomedical Engineering, Zhejiang University, Hangzhou, China
Bioengineering College, Zhejiang Chinese Medical University, Hangzhou, China
Pharmaceutical College, Zhejiang Chinese Medical University, Hangzhou, China
Received November 14, 2014
Abstract—Preparations based on medicinal herbs used in the traditional Chinese medicine to cure and make a
prophylactic treatment of a wide variety of diseases wee studied. The goal of the study was to assess the capacity
of a potentiometric multisensor system of the electronic-tongue type in an instrumental evaluation of the toxicity of
samples being analyzed. The toxicity of herb preparations was evaluated by the cell index calculated from results
of an analysis of these preparations by biosensors. A procedure for measurement of samples with the multisensor
system was developed. Mathematical models relating the values obtained to reference data were constructed. The
parameters of these models suggest that the potentiometric multisensor system can prognosticate the toxicity of
samples under study with a relative error not exceeding 11%.
Moder pharmacology has accumulated a colossal
experience of application of preparations based on the
traditional Chinese medicine (TCM) [1–5]. At present,
the TCM is a combination of the conventional knowledge
about properties of herb preparation with the recent
scientific achievements. It is believed that just this
approach has made it possible to raise the average life
expectancy in China from 50 to 73 years.
The experience of application of medicinal preparations
has been accumulated in China during several millennia.
It has been possible to determine the curing effect of
approximately 200 medicinal herb species . It is
believed that, for the medicinal preparations used in the
TCM, the effect on the organism of a patient is slower
than that of modern chemotherapeutic preparations.
It is important to note that a whole complex of herb
components is responsible for the effect of medicals of
this kind, rather than a single component (drug) common
for the Western medicine. Table 1 presents the classes
of substances most frequently determined in TCM
The number of scientiﬁ c preparations concerned with
TCM preparations has strongly increased during the last
20 years (Fig. 1).
The most frequent are publications devoted to studies
of separate components contained in herbs to reveal their
biological activity. To solve this problem, it is necessary
to have selective methods with low detection limits of
bioactive compounds of this kind . The most frequently
employed techniques are those of chromatography and
capillary electrophoresis in combination with various
detection and concentration methods [8–15].
It should be noted that the sample preparation for an
analysis of this kind is complicated. The conventional
sample preparation procedures include the shredding of a
dried or frozen sample, homogenization, extraction with
a solvent, centrifugation, and ﬁ ltration .