Determination of the duration of a primary immune response and the ID 50 of ALA rabbit rotavirus in rabbits

Determination of the duration of a primary immune response and the ID 50 of ALA rabbit rotavirus... The rabbit model of rotavirus infection has been used to examine the immune response to rotavirus infection and to evaluate strategies for rotavirus vaccine development. To determine the 50% infectious does (ID 50 ) of tissue culture adapted ALA virus, rabbits were orally inoculated with 10 1 –10 3 PFU of ALA rotavirus. The ID 50 of ALA virus was determined to be 1.7×10 2 PFU. The immune response induced in rabbits infected at low virus doses (10 2 –10 3 PFU) was of similar magnitude to the immune responses induced with a high dose (10 6 PFU) inoculum, indicating that the immune response to ALA rotavirus in rabbits is not dose dependent. To determine if a single rotavirus inoculation would induce a long lasting immune response, four rabbits were inoculated once with ALA virus (3.5 × 10 5 PFU) and their serologic and mucosal antirotavirus titers were monitored at intervals for 1.5–2 years. The infected rabbits maintained serologic and mucosal rotavirus antibody titers until the final time point more than 700 days post inoculation. These data are important because they indicate that the antigenic load achieved following a single oral inoculation is sufficient to achieve long lasting immunity, the goal of any potential vaccine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Determination of the duration of a primary immune response and the ID 50 of ALA rabbit rotavirus in rabbits

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Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1997 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050242
Publisher site
See Article on Publisher Site

Abstract

The rabbit model of rotavirus infection has been used to examine the immune response to rotavirus infection and to evaluate strategies for rotavirus vaccine development. To determine the 50% infectious does (ID 50 ) of tissue culture adapted ALA virus, rabbits were orally inoculated with 10 1 –10 3 PFU of ALA rotavirus. The ID 50 of ALA virus was determined to be 1.7×10 2 PFU. The immune response induced in rabbits infected at low virus doses (10 2 –10 3 PFU) was of similar magnitude to the immune responses induced with a high dose (10 6 PFU) inoculum, indicating that the immune response to ALA rotavirus in rabbits is not dose dependent. To determine if a single rotavirus inoculation would induce a long lasting immune response, four rabbits were inoculated once with ALA virus (3.5 × 10 5 PFU) and their serologic and mucosal antirotavirus titers were monitored at intervals for 1.5–2 years. The infected rabbits maintained serologic and mucosal rotavirus antibody titers until the final time point more than 700 days post inoculation. These data are important because they indicate that the antigenic load achieved following a single oral inoculation is sufficient to achieve long lasting immunity, the goal of any potential vaccine.

Journal

Archives of VirologySpringer Journals

Published: Nov 1, 1997

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