Determination of Functional Role of Nucleotide Composition in the Transcription Start Region of the Escherichia coli udp Gene

Determination of Functional Role of Nucleotide Composition in the Transcription Start Region of... The promoter of the Escherichia coli udp gene contains the poly-T (5"-TTTTT-3") motif in the transcription start region located at the distance of 3 nucleotides with respect to the Pribnow box. By means of site-directed mutagenesis, mutations in the +1, –1, and +3 positions of this region were isolated and their functional role in transcription initiation was determined. It was shown that in addition to the thymine nucleotide earlier identified at position 4 (with respect to the 5" end of the poly-T motif), the third thymine nucleotide may serve as an efficient transcription site. The functional significance of the presence of the poly-T motif in the transcription initiation region is discussed. Analysis of the isolated mutant promoters revealed the ability of the guanine nucleotide at the +3 position to stabilize the formation of a productive transcription complex and to serve as an additional transcription start. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Determination of Functional Role of Nucleotide Composition in the Transcription Start Region of the Escherichia coli udp Gene

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1023204624319
Publisher site
See Article on Publisher Site

Abstract

The promoter of the Escherichia coli udp gene contains the poly-T (5"-TTTTT-3") motif in the transcription start region located at the distance of 3 nucleotides with respect to the Pribnow box. By means of site-directed mutagenesis, mutations in the +1, –1, and +3 positions of this region were isolated and their functional role in transcription initiation was determined. It was shown that in addition to the thymine nucleotide earlier identified at position 4 (with respect to the 5" end of the poly-T motif), the third thymine nucleotide may serve as an efficient transcription site. The functional significance of the presence of the poly-T motif in the transcription initiation region is discussed. Analysis of the isolated mutant promoters revealed the ability of the guanine nucleotide at the +3 position to stabilize the formation of a productive transcription complex and to serve as an additional transcription start.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 7, 2004

References

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