Hemoglobin–stabilized gold nanoclusters (Hb–AuNCs) were prepared by using hemoglobin as both reducing and stabilizing agents. The Hb–AuNCs display a strong catalytic effect on the chemiluminescence (CL) reaction of luminol with NaIO4. The CL mechanism is discussed and the experimental variables are examined. It is found that dopamine strongly inhibits CL. This finding is exploited in a CL–based dopamine assay that works in the range between 0.3 and 9.0 nM and has a 0.1 nM detection limit. The relative standard deviation is 3.1% at a 5 nM dopamine level (for n = 11). The method was applied to the determination of dopamine in spiked human plasma with satisfactory results.
Microchimica Acta – Springer Journals
Published: Jun 27, 2017
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