Arch Virol (1999) 144: 1679–1687
Determination of 3
-terminal nucleotide sequence of pepper
vein banding virus RNA and expression of its coat protein
in Escherichia coli
J. Joseph and H. S. Savithri
Department of Biochemistry, Indian Institute of Science, Bangalore, India
Accepted April 28, 1999
Summary. Pepper vein banding virus (PVBV) is an important virus infecting
chilli pepper in south India. Earlier reports suggested it to be a distinct potyvirus.
The nucleotide sequence of PVBV RNA from the 3
-end (3862 nt) was deter-
mined. Analysis of the nucleotide and deduced amino acid sequence revealed
that it encompasses a partial open reading frame encoding the partial sequence
of VPg, NIa-protease, NIb, coat protein (CP) and 3
-untranslated region (UTR).
Comparison of the amino acid sequence of CP and the nucleotide sequence of
-UTR with those of other potyviruses conﬁrmed an earlier observation that
PVBV is a distinct member of the Potyvirus sub-group and it had signiﬁcant
similarity to a recently characterized virus infecting chilli pepper, chilli vein-
banding mottle virus (CVbMV), from Thailand. The analysis showed that both
PVBV and CVbMV might represent strains of the same virus. Further, the PVBV
CP gene was overexpressed in E. coli, which assembled into potyvirus-like parti-
cles (PVLPs). The assembled particles were shown to encapsidate the CP mRNA.
Papper vein banding virus has been reported to be the most prevalent virus infect-
ing chilli pepper in south India . Initial characterization of PVBV with respect
to particle morphology, serology and N-terminal amino acid sequence of the coat
protein (CP) suggested it to be a distinct member of the Potyvirus sub-group .
However, complete amino acid sequence of the CP and the nucleotide sequence
at the s3
-untranslated region provide a better basis for the classiﬁcation of po-
tyviruses [8, 32]. In addition, molecular characterization is an important aspect in
The nucleotide sequence reported in this paper has been submitted to EMBL nucleotide
sequence database and assigned the accession number AJ237843.