Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR

Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR Current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. Specific primers to detect the presence of viral genomes were developed using an SYBR-Green-based real-time RT-PCR protocol. One hundred sixty-four rodents native to the Atlantic Forest biome were captured in São Paulo State, Brazil, and their tissues were tested. The presence of hantavirus RNA was detected in sixteen rodents: three specimens of Akodon montensis , three of Akodon cursor , two of Necromys lasiurus , one of Juliomys sp., one of Thaptomys nigrita , five of Oligoryzomys nigripes , and one of Oryzomys sp. This SYBR Green real-time RT-PCR method for detection of hantavirus may be useful for surveying hantaviruses in Brazil. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR

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Publisher
Springer Vienna
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Biomedicine; Medical Microbiology ; Infectious Diseases; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-011-0968-1
Publisher site
See Article on Publisher Site

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