Detection of DNA Mismatch Repair Protein Abnormalities in Sudanese Colorectal Cancer Patients Using Immunohistochemical Methods

Detection of DNA Mismatch Repair Protein Abnormalities in Sudanese Colorectal Cancer Patients... Purpose DNA mismatch repair (MMR) protein abnormalities among 42 Sudanese colorectal cancer (CRC) patients were assessed. Methods Sections were stained by immunohistochemical method to assess the abnormalities of MLH1, MSH2, MSH6, and PMS2. Results Of the 42 included cases, 34 (80.95%) were MMR protein positive for all MMR proteins under assessment, 3 (7.14%) MSH2 inadequate, and 1 (2.38%) MSH6 inadequate. Abnormal MMR protein expression was found in 4 (9.5%) cases. Of these, 2 (50%) were MSH2 and MSH6 negative and 2 (50%) were MLH1 and PMS2 negative. Regarding microsatellite instability (MSI) results, the three cases that were MSH2 inadequate and positive for the rest by immunohistochemistry (IHC) showed stable results with both BAT 25 and 26. The case that was MSH6 inadequate showed stable results with both BAT 25 and 26. The 2 cases with MSH2- and MSH6-negative results were unstable with both BAT 25 and 26. Of the two cases that were MLH1 and PMS2 negative, one case showed non-evaluable results with both BAT 25 and 26 while the other case was unstable with BAT 26 and not evaluable with BAT 25. Conclusions The percentage of MMR protein-negative cases in Sudanese CRC patients appears to be relatively low http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Gastrointestinal Cancer Springer Journals

Detection of DNA Mismatch Repair Protein Abnormalities in Sudanese Colorectal Cancer Patients Using Immunohistochemical Methods

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Medicine & Public Health; Oncology; Gastroenterology; Radiotherapy; Cancer Research; Internal Medicine
ISSN
1941-6628
eISSN
1941-6636
D.O.I.
10.1007/s12029-018-0118-z
Publisher site
See Article on Publisher Site

Abstract

Purpose DNA mismatch repair (MMR) protein abnormalities among 42 Sudanese colorectal cancer (CRC) patients were assessed. Methods Sections were stained by immunohistochemical method to assess the abnormalities of MLH1, MSH2, MSH6, and PMS2. Results Of the 42 included cases, 34 (80.95%) were MMR protein positive for all MMR proteins under assessment, 3 (7.14%) MSH2 inadequate, and 1 (2.38%) MSH6 inadequate. Abnormal MMR protein expression was found in 4 (9.5%) cases. Of these, 2 (50%) were MSH2 and MSH6 negative and 2 (50%) were MLH1 and PMS2 negative. Regarding microsatellite instability (MSI) results, the three cases that were MSH2 inadequate and positive for the rest by immunohistochemistry (IHC) showed stable results with both BAT 25 and 26. The case that was MSH6 inadequate showed stable results with both BAT 25 and 26. The 2 cases with MSH2- and MSH6-negative results were unstable with both BAT 25 and 26. Of the two cases that were MLH1 and PMS2 negative, one case showed non-evaluable results with both BAT 25 and 26 while the other case was unstable with BAT 26 and not evaluable with BAT 25. Conclusions The percentage of MMR protein-negative cases in Sudanese CRC patients appears to be relatively low

Journal

Journal of Gastrointestinal CancerSpringer Journals

Published: May 31, 2018

References

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