Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey

Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey Bovine torovirus (BToV), a member of the family Coronaviridae , is an established gastrointestinal infectious agent in cattle. In this study, we performed a survey to detect BToV in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by the nested reverse transcription (RT) PCR method using primers located in the consensus sequences of the BToV membrane (M) gene. The BToV M gene was detected in 4.7 % (11/235) of the samples using the nested RT-PCR method. The nucleotide sequences of partial M fragments from the BToV isolates, including the newly identified Turkish isolates, showed more than 96 % identity. The result indicates that BToV is one of the pathogens that contribute to neonatal calf diarrhea cases in Turkey. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-1977-7
Publisher site
See Article on Publisher Site

Abstract

Bovine torovirus (BToV), a member of the family Coronaviridae , is an established gastrointestinal infectious agent in cattle. In this study, we performed a survey to detect BToV in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by the nested reverse transcription (RT) PCR method using primers located in the consensus sequences of the BToV membrane (M) gene. The BToV M gene was detected in 4.7 % (11/235) of the samples using the nested RT-PCR method. The nucleotide sequences of partial M fragments from the BToV isolates, including the newly identified Turkish isolates, showed more than 96 % identity. The result indicates that BToV is one of the pathogens that contribute to neonatal calf diarrhea cases in Turkey.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 2014

References

  • The complete sequence of the bovine torovirus genome
    Draker, R; Roper, RL; Petric, M; Tellier, R
  • Bovine torovirus: sequencing of the structural genes and expression of the nucleocapsid protein of Breda virus
    Duckmanton, LM; Tellier, R; Liu, P; Petric, M
  • A comparative sequence analysis to revise the current taxonomy of the family Coronaviridae
    Gonzalez, JM; Gomez-Puertas, P; Cavanagh, D; Gorbalenya, AE; Enjuanes, L

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