Detection and partial molecular characterization of atypical plum pox virus isolates from naturally infected sour cherry

Detection and partial molecular characterization of atypical plum pox virus isolates from... Atypical isolates of plum pox virus (PPV) were discovered in naturally infected sour cherry in urban ornamental plantings in Moscow, Russia. The isolates were detected by polyclonal double antibody sandwich ELISA and RT-PCR using universal primers specific for the 3′-non-coding and coat protein (CP) regions of the genome but failed to be recognized by triple antibody sandwich ELISA with the universal monoclonal antibody 5B and by RT-PCR using primers specific to for PPV strains D, M, C and W. Sequence analysis of the CP genes of nine isolates revealed 99.2-100 % within-group identity and 62-85 % identity to conventional PPV strains. Phylogenetic analysis showed that the atypical isolates represent a group that is distinct from the known PPV strains. Alignment of the N-terminal amino acid sequences of CP demonstrated their close similarity to those of a new tentative PPV strain, CR. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Detection and partial molecular characterization of atypical plum pox virus isolates from naturally infected sour cherry

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1630-x
Publisher site
See Article on Publisher Site

Abstract

Atypical isolates of plum pox virus (PPV) were discovered in naturally infected sour cherry in urban ornamental plantings in Moscow, Russia. The isolates were detected by polyclonal double antibody sandwich ELISA and RT-PCR using universal primers specific for the 3′-non-coding and coat protein (CP) regions of the genome but failed to be recognized by triple antibody sandwich ELISA with the universal monoclonal antibody 5B and by RT-PCR using primers specific to for PPV strains D, M, C and W. Sequence analysis of the CP genes of nine isolates revealed 99.2-100 % within-group identity and 62-85 % identity to conventional PPV strains. Phylogenetic analysis showed that the atypical isolates represent a group that is distinct from the known PPV strains. Alignment of the N-terminal amino acid sequences of CP demonstrated their close similarity to those of a new tentative PPV strain, CR.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2013

References

  • Further characterization of a new recombinant group of plum pox virus isolates, PPV-T, found in orchards in the Ankara province of Turkey
    Serce, CU; Candresse, T; Svanella-Dumas, L; Krizbai, L; Gazela, M; Caglayan, K
  • Hosts and symptoms of plum pox virus: fruiting Prunus species
    Llácer, G; Cambra, M
  • Hosts and symptoms of plum pox virus: ornamental and wild Prunus species
    James, D; Thompson, D
  • Plum pox virus (PPV) in sweet cherry
    Crescenzi, A; Nuzzaci, M; Levy, L; Piazzolla, P; Hadidi, A

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