Detection and molecular characterization of bovine leukemia virus in Philippine cattle

Detection and molecular characterization of bovine leukemia virus in Philippine cattle Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. However, there are no comprehensive studies on the distribution of BLV in the Philippines, and the genetic characteristics of Philippine BLV strains are unknown. Therefore, the aim of this study was to detect BLV infections in the Philippines and determined their genetic variability. Blood samples were obtained from 1116 cattle from different farms on five Philippine islands, and BLV provirus was detected by BLV-CoCoMo-qPCR-2 and nested PCR targeting BLV long terminal repeats. Out of 1116 samples, 108 (9.7 %) and 54 (4.8 %) were positive for BLV provirus, as determined by BLV-CoCoMo-qPCR-2 and nested PCR, respectively. Of the five islands, Luzon Island showed the highest prevalence of BLV infection (23.1 %). Partial env gp51 genes from 43 samples, which were positive for BLV provirus by both methods, were sequenced for phylogenetic analysis. Phylogenetic analysis based on a 423-bp fragment of the env gene revealed that Philippine BLV strains clustered into either genotype 1 or genotype 6. Substitutions were mainly found in antigenic determinants, such as the CD4 + T-cell epitope, the CD8 + T-cell epitope, the second neutralizing domain, B and E epitopes, and these substitutions varied according to genotype. This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Detection and molecular characterization of bovine leukemia virus in Philippine cattle

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2280-3
Publisher site
See Article on Publisher Site

Abstract

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. However, there are no comprehensive studies on the distribution of BLV in the Philippines, and the genetic characteristics of Philippine BLV strains are unknown. Therefore, the aim of this study was to detect BLV infections in the Philippines and determined their genetic variability. Blood samples were obtained from 1116 cattle from different farms on five Philippine islands, and BLV provirus was detected by BLV-CoCoMo-qPCR-2 and nested PCR targeting BLV long terminal repeats. Out of 1116 samples, 108 (9.7 %) and 54 (4.8 %) were positive for BLV provirus, as determined by BLV-CoCoMo-qPCR-2 and nested PCR, respectively. Of the five islands, Luzon Island showed the highest prevalence of BLV infection (23.1 %). Partial env gp51 genes from 43 samples, which were positive for BLV provirus by both methods, were sequenced for phylogenetic analysis. Phylogenetic analysis based on a 423-bp fragment of the env gene revealed that Philippine BLV strains clustered into either genotype 1 or genotype 6. Substitutions were mainly found in antigenic determinants, such as the CD4 + T-cell epitope, the CD8 + T-cell epitope, the second neutralizing domain, B and E epitopes, and these substitutions varied according to genotype. This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2015

References

  • Molecular characterization of the env gene from Brazilian field isolates of Bovine leukemia virus
    Camargos, MF; Pereda, A; Stancek, D; Rocha, MA; Reis, JK; Greiser-Wilke, I; Leite, RC
  • Molecular analysis of a 444 bp fragment of the bovine leukaemia virus gp51 env gene reveals a high frequency of non-silent point mutations and suggests the presence of two subgroups of BLV in Chile
    Felmer, R; Munoz, G; Zuniga, J; Recabal, M
  • Genetic diversity and spread of Bovine leukaemia virus isolates in Argentine dairy cattle
    Monti, G; Schrijver, R; Beier, D
  • Identification of a new genotype of bovine leukemia virus
    Balic, D; Lojkic, I; Periskic, M; Bedekovic, T; Jungic, A; Lemo, N; Roic, B; Cac, Z; Barbic, L; Madic, J
  • Phylogenetic analysis of bovine leukemia viruses isolated in South America reveals diversification in seven distinct genotypes
    Moratorio, G; Obal, G; Dubra, A; Correa, A; Bianchi, S; Buschiazzo, A; Cristina, J; Pritsch, O
  • Distribution and superinfection of bovine leukemia virus genotypes in Japan
    Asfaw, Y; Tsuduku, S; Konishi, M; Murakami, K; Tsuboi, T; Wu, D; Sentsui, H
  • Sequencing and phylogenetic analysis of gp51 gene of bovine leukaemia virus in Iranian isolates
    Hemmatzadeh, F
  • Genetic heterogeneity among bovine leukemia virus genotypes and its relation to humoral responses in hosts
    Licursi, M; Inoshima, Y; Wu, D; Yokoyama, T; Gonzalez, ET; Sentsui, H

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