Detection and characterisation of two novel vitiviruses infecting Actinidia

Detection and characterisation of two novel vitiviruses infecting Actinidia Two co-infecting novel vitiviruses from Actinidia chinensis were identified from mechanically inoculated Nicotiana occidentalis . Both virus genomes were sequenced and share 64% nucleotide identity. Their overall structure is typical of vitiviruses, with five open reading frames (ORFs) and a polyadenylated 3′ end. Open reading frame 4 (ORF4) encodes the coat protein, the most conserved gene of the vitiviruses, in which they share 75% amino acid identity, 61-68% with grapevine virus B, 55-59% with grapevine virus A, and 37-42% with grapevine virus E. Based on the molecular criteria for species demarcation in the family Betaflexiviridae , these are two novel viruses, tentatively named Actinidia virus A and Actinidia virus B. Archives of Virology Springer Journals

Detection and characterisation of two novel vitiviruses infecting Actinidia

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Springer Vienna
Copyright © 2012 by Springer-Verlag
Biomedicine; Infectious Diseases; Medical Microbiology; Virology
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