Description of a novel single mutation in the Ac MNPV polyhedrin gene that results in abnormally large cubic polyhedra

Description of a novel single mutation in the Ac MNPV polyhedrin gene that results in abnormally... We describe a point mutation in the Ac MNPV polyhedrin gene that produces abnormally large cubic polyhedra in packaging cell lines. A polyhedrin mutant baculovirus in which the single change E44G was introduced confirmed that this mutation and no other alterations in the Ac MNPV genome was responsible for the abnormal phenotype. Although baculoviral VP39 protein was detected inside mutant polyhedra, electron microscopy demonstrated that only a proportion of the large crystals allow occlusion of virions. When compared with wild-type polyhedra, the mutant inoculum showed reduced oral infectivity for Rachiplusia nu larvae. Hence, the amino acid 44 substitution in the Ac MNPV polyhedrin protein alters polyhedrin assembly and affects viral occlusion and infectivity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Description of a novel single mutation in the Ac MNPV polyhedrin gene that results in abnormally large cubic polyhedra

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Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-010-0885-8
Publisher site
See Article on Publisher Site

Abstract

We describe a point mutation in the Ac MNPV polyhedrin gene that produces abnormally large cubic polyhedra in packaging cell lines. A polyhedrin mutant baculovirus in which the single change E44G was introduced confirmed that this mutation and no other alterations in the Ac MNPV genome was responsible for the abnormal phenotype. Although baculoviral VP39 protein was detected inside mutant polyhedra, electron microscopy demonstrated that only a proportion of the large crystals allow occlusion of virions. When compared with wild-type polyhedra, the mutant inoculum showed reduced oral infectivity for Rachiplusia nu larvae. Hence, the amino acid 44 substitution in the Ac MNPV polyhedrin protein alters polyhedrin assembly and affects viral occlusion and infectivity.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2011

References

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