Deoxyribonucleic Acid Barcoding of Some Indian Coelogyninae (Orchidaceae)

Deoxyribonucleic Acid Barcoding of Some Indian Coelogyninae (Orchidaceae) Subtribe Coelogyninae (Epidendroideae, Orchidaceae) comprises of 16 genera. The present study is undertaken to develop DNA barcodes of subtribe Coelogyninae using rbcL. It reveals the sectional delineation and its relationship with Pholidota. DNA barcoding is a technique which helps identification of species even if only small fragment of organism is available at any stage. The rbcL (Rubisco large subunit) from chloroplast genome is tested for effective barcoding. The interspecific divergence values and species discrimination rates were calculated by Kimura 2 parameter using MEGA 4.0. The rbcL with average interspecific divergence values yielded 72.72 % species resolution, thus it could distinguish all the species of Coelogyne and Pholidota. Invariably the genus Pholidota shows close affinity with Coelogyne. This supports the inclusion of genus Pholidota in the subtribe Coelogyninae of tribe Coelogyneae. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Deoxyribonucleic Acid Barcoding of Some Indian Coelogyninae (Orchidaceae)

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Publisher
Springer Journals
Copyright
Copyright © 2016 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
D.O.I.
10.1007/s40011-016-0756-5
Publisher site
See Article on Publisher Site

Abstract

Subtribe Coelogyninae (Epidendroideae, Orchidaceae) comprises of 16 genera. The present study is undertaken to develop DNA barcodes of subtribe Coelogyninae using rbcL. It reveals the sectional delineation and its relationship with Pholidota. DNA barcoding is a technique which helps identification of species even if only small fragment of organism is available at any stage. The rbcL (Rubisco large subunit) from chloroplast genome is tested for effective barcoding. The interspecific divergence values and species discrimination rates were calculated by Kimura 2 parameter using MEGA 4.0. The rbcL with average interspecific divergence values yielded 72.72 % species resolution, thus it could distinguish all the species of Coelogyne and Pholidota. Invariably the genus Pholidota shows close affinity with Coelogyne. This supports the inclusion of genus Pholidota in the subtribe Coelogyninae of tribe Coelogyneae.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological SciencesSpringer Journals

Published: Jun 27, 2016

References

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