Denaturation and Aggregation of Interferon-τ in Aqueous Solution

Denaturation and Aggregation of Interferon-τ in Aqueous Solution Pharm Res (2018) 35: 137 https://doi.org/10.1007/s11095-018-2418-1 RESEARCH PAPER Denaturation and Aggregation of Interferon-τ in Aqueous Solution 1 2 3,4 3 3,4 3,4 Ryan R. Manning & Glenn A. Wilson & Ryan E. Holcomb & Nathaniel J. Zbacnik & Auria A. Tellechea & Chelsey L. Gilley-Dunn & 3 3 3 4 3,4 5 Ryan J. Krammes & Nathan S. Krammes & Gabriel J. Evans & Charles S. Henry & Mark Cornell Manning & Brian M. Murphy & 3,4 3,4 Robert W. Payne & Derrick S. Katayama Received: 24 February 2018 /Accepted: 22 April 2018 /Published online: 9 May 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 ABSTRACT at 50°C. Finally, the thermal aggregation was monitored Purpose To evaluate the different degrees of residual struc- using AF4 and the rate constants were found to be compara- ture in the unfolded state of interferon-τ using chemical dena- ble to those determined previously by SEC and DLS. The turation as a function of temperature by both urea and thermal aggregation appears to be consistent with a guanidinium hydrochloride. nucleation-dependent mechanism with a critical nucleus size Methods Asymmetrical flow field-flow fractionation (AF4) of 4 ± 1. using both UV and multi-angle http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pharmaceutical Research Springer Journals

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Biomedicine; Pharmacology/Toxicology; Pharmacy; Biochemistry, general; Medical Law; Biomedical Engineering
ISSN
0724-8741
eISSN
1573-904X
D.O.I.
10.1007/s11095-018-2418-1
Publisher site
See Article on Publisher Site

Abstract

Pharm Res (2018) 35: 137 https://doi.org/10.1007/s11095-018-2418-1 RESEARCH PAPER Denaturation and Aggregation of Interferon-τ in Aqueous Solution 1 2 3,4 3 3,4 3,4 Ryan R. Manning & Glenn A. Wilson & Ryan E. Holcomb & Nathaniel J. Zbacnik & Auria A. Tellechea & Chelsey L. Gilley-Dunn & 3 3 3 4 3,4 5 Ryan J. Krammes & Nathan S. Krammes & Gabriel J. Evans & Charles S. Henry & Mark Cornell Manning & Brian M. Murphy & 3,4 3,4 Robert W. Payne & Derrick S. Katayama Received: 24 February 2018 /Accepted: 22 April 2018 /Published online: 9 May 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 ABSTRACT at 50°C. Finally, the thermal aggregation was monitored Purpose To evaluate the different degrees of residual struc- using AF4 and the rate constants were found to be compara- ture in the unfolded state of interferon-τ using chemical dena- ble to those determined previously by SEC and DLS. The turation as a function of temperature by both urea and thermal aggregation appears to be consistent with a guanidinium hydrochloride. nucleation-dependent mechanism with a critical nucleus size Methods Asymmetrical flow field-flow fractionation (AF4) of 4 ± 1. using both UV and multi-angle

Journal

Pharmaceutical ResearchSpringer Journals

Published: May 9, 2018

References

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