Pharm Res (2018) 35: 137 https://doi.org/10.1007/s11095-018-2418-1 RESEARCH PAPER Denaturation and Aggregation of Interferon-τ in Aqueous Solution 1 2 3,4 3 3,4 3,4 Ryan R. Manning & Glenn A. Wilson & Ryan E. Holcomb & Nathaniel J. Zbacnik & Auria A. Tellechea & Chelsey L. Gilley-Dunn & 3 3 3 4 3,4 5 Ryan J. Krammes & Nathan S. Krammes & Gabriel J. Evans & Charles S. Henry & Mark Cornell Manning & Brian M. Murphy & 3,4 3,4 Robert W. Payne & Derrick S. Katayama Received: 24 February 2018 /Accepted: 22 April 2018 /Published online: 9 May 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 ABSTRACT at 50°C. Finally, the thermal aggregation was monitored Purpose To evaluate the different degrees of residual struc- using AF4 and the rate constants were found to be compara- ture in the unfolded state of interferon-τ using chemical dena- ble to those determined previously by SEC and DLS. The turation as a function of temperature by both urea and thermal aggregation appears to be consistent with a guanidinium hydrochloride. nucleation-dependent mechanism with a critical nucleus size Methods Asymmetrical flow field-flow fractionation (AF4) of 4 ± 1. using both UV and multi-angle
Pharmaceutical Research – Springer Journals
Published: May 9, 2018
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