Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory syndrome virus replication

Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory... The macrophage scavenger receptor CD163 is a major determinant for the entry of porcine reproductive and respiratory syndrome virus (PRRSV). The present study was undertaken to assess the functional significance of the intracellular region of CD163 for PRRSV infection. We stably transfected non-susceptible BHK cells with plasmids encoding full-length and cytoplasmic-domain-deleted (tailless) CD163 and evaluated their permissiveness to PRRSV. The experimental data revealed that the tailless CD163 was not only completely sufficient for converting non-target cells to PRRSV susceptibility, but it also notably enhanced virus replication, with significant increases in viral protein synthesis and progeny release. Taken together, our results suggest that the intracellular domain of CD163 may be associated with an important yet-unknown function during PRRSV infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory syndrome virus replication

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Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-010-0699-8
Publisher site
See Article on Publisher Site

Abstract

The macrophage scavenger receptor CD163 is a major determinant for the entry of porcine reproductive and respiratory syndrome virus (PRRSV). The present study was undertaken to assess the functional significance of the intracellular region of CD163 for PRRSV infection. We stably transfected non-susceptible BHK cells with plasmids encoding full-length and cytoplasmic-domain-deleted (tailless) CD163 and evaluated their permissiveness to PRRSV. The experimental data revealed that the tailless CD163 was not only completely sufficient for converting non-target cells to PRRSV susceptibility, but it also notably enhanced virus replication, with significant increases in viral protein synthesis and progeny release. Taken together, our results suggest that the intracellular domain of CD163 may be associated with an important yet-unknown function during PRRSV infection.

Journal

Archives of VirologySpringer Journals

Published: Aug 1, 2010

References

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