Deletion analysis of the sapovirus VP1 gene for the assembly of virus-like particles

Deletion analysis of the sapovirus VP1 gene for the assembly of virus-like particles Human sapovirus (SaV) strains are agents of gastroenteritis. They cannot be grown in cell culture. In this study, constructs containing SaV N- and C-terminal-deleted recombinant capsid proteins (rVP1) were expressed in a baculovirus expression system to allow us to better understand the sequence requirements for the formation of virus-like particles (VLPs). Only proteins derived from N-terminal-deleted rVP1 constructs that began 49 nucleotides downstream assembled into VLPs, which included both small and native-size VLPs. Our results were similar to those reported in a rabbit hemorrhagic disease virus (RHDV) N- and C-terminal-deleted rVP1 expression study but were distinct from those reported in a norovirus N- and C-terminal-deleted rVP1 expression study, suggesting that SaV and RHDV may have similar expression requirements. Archives of Virology Springer Journals

Deletion analysis of the sapovirus VP1 gene for the assembly of virus-like particles

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Copyright © 2005 by Springer-Verlag/Wien
Biomedicine; Medical Microbiology; Infectious Diseases; Virology
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