Definition of an epitope on Japanese encephalitis virus (JEV) envelope protein recognized by JEV-specific murine CD8 + cytotoxic T lymphocytes

Definition of an epitope on Japanese encephalitis virus (JEV) envelope protein recognized by... We defined an epitope on the Japanese encephalitis virus (JEV) envelope (E) protein recognized by CD8 + cytotoxic T lymphocytes (CTLs). CTLs induced in JEV-infected BALB/c (H-2 d ) mice recognized E and/or premembrane (PrM) proteins, while CTLs in C57BL/6J (H-2 b ) and C3H/HeJ (H-2 k ) mice did not. JEV-specific CTLs had a phenotype of CD3 + CD4 − CD8 + . Twenty-four 9-amino acid (a.a.) peptides, which had binding motifs for H-2K d , H-2L d or H-2D d , were synthesized according to the amino acid sequences of PrM and E proteins. CTLs induced by JEV infection recognized only the peptide K-3. Immunization of BALB/c mice with only a group of peptides including K-3 induced CTLs which recognized the homologous K-3 peptide, while immunization with other peptides did not. The peptide K-3 had a binding motif for H-2K d . This is consistent with the finding that JEV-specific CTLs in BALB/c mice was H-2K d -restricted. These results indicate that the epitope recognized by CTLs in BALB/c mice is located between a.a. 60 and 68 on the E protein, corresponding to an a.a. sequence of CYHASVTDI. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Definition of an epitope on Japanese encephalitis virus (JEV) envelope protein recognized by JEV-specific murine CD8 + cytotoxic T lymphocytes

Loading next page...
 
/lp/springer_journal/definition-of-an-epitope-on-japanese-encephalitis-virus-jev-envelope-Y8FHhOiDaQ
Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050043
Publisher site
See Article on Publisher Site

Abstract

We defined an epitope on the Japanese encephalitis virus (JEV) envelope (E) protein recognized by CD8 + cytotoxic T lymphocytes (CTLs). CTLs induced in JEV-infected BALB/c (H-2 d ) mice recognized E and/or premembrane (PrM) proteins, while CTLs in C57BL/6J (H-2 b ) and C3H/HeJ (H-2 k ) mice did not. JEV-specific CTLs had a phenotype of CD3 + CD4 − CD8 + . Twenty-four 9-amino acid (a.a.) peptides, which had binding motifs for H-2K d , H-2L d or H-2D d , were synthesized according to the amino acid sequences of PrM and E proteins. CTLs induced by JEV infection recognized only the peptide K-3. Immunization of BALB/c mice with only a group of peptides including K-3 induced CTLs which recognized the homologous K-3 peptide, while immunization with other peptides did not. The peptide K-3 had a binding motif for H-2K d . This is consistent with the finding that JEV-specific CTLs in BALB/c mice was H-2K d -restricted. These results indicate that the epitope recognized by CTLs in BALB/c mice is located between a.a. 60 and 68 on the E protein, corresponding to an a.a. sequence of CYHASVTDI.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2000

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off