We defined an epitope on the Japanese encephalitis virus (JEV) envelope (E) protein recognized by CD8 + cytotoxic T lymphocytes (CTLs). CTLs induced in JEV-infected BALB/c (H-2 d ) mice recognized E and/or premembrane (PrM) proteins, while CTLs in C57BL/6J (H-2 b ) and C3H/HeJ (H-2 k ) mice did not. JEV-specific CTLs had a phenotype of CD3 + CD4 − CD8 + . Twenty-four 9-amino acid (a.a.) peptides, which had binding motifs for H-2K d , H-2L d or H-2D d , were synthesized according to the amino acid sequences of PrM and E proteins. CTLs induced by JEV infection recognized only the peptide K-3. Immunization of BALB/c mice with only a group of peptides including K-3 induced CTLs which recognized the homologous K-3 peptide, while immunization with other peptides did not. The peptide K-3 had a binding motif for H-2K d . This is consistent with the finding that JEV-specific CTLs in BALB/c mice was H-2K d -restricted. These results indicate that the epitope recognized by CTLs in BALB/c mice is located between a.a. 60 and 68 on the E protein, corresponding to an a.a. sequence of CYHASVTDI.
Archives of Virology – Springer Journals
Published: Mar 1, 2000
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.
All for just $49/month
Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.
Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.
It’s easy to organize your research with our built-in tools.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera