The MADS proteins APETALA3 (AP3), PISTILLATA (PI), SEPALLATA1 (SEP1), SEP2, SEP3, AGAMOUS, and APETALA1 are required for proper ﬂoral organ identity in Arabidopsis ﬂowers. All of these ﬂoral MADS proteins conserve two domains: the MADS domain that mediates DNA binding and dimerization, and the K domain that mediates protein–protein interaction. The K domain is postulated to form a several amphipathic a-helices referred to as K1, K2, and K3. The K1 and K2 helicies are located entirely within the K domain while the K3 helix spans the K domain-C domain boundary. Here we report on our studies on the interactions of the B class MADS proteins AP3 and PI with the E class MADS proteins SEP1, SEP2, and SEP3. A comparative analysis of mutants in the K domain reveals that the subdomains mediating the PI/AP3 interaction are diﬀerent from the subdomains mediating the PI/SEP3 (or PI/SEP1) interaction. The strong PI/SEP3 (or PI/SEP1) interaction requires K2, part of K3, and the interhelical region between K1 and K2. By contrast, K1, K2 and the region between K1 and K2 are important for strong AP3/PI interaction. Most of the K3 helix does not appear to be important for either the PI/AP3 or the
Plant Molecular Biology – Springer Journals
Published: Dec 30, 2004
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