Defining subdomains of the K domain important for protein–protein interactions of plant MADS proteins

Defining subdomains of the K domain important for protein–protein interactions of plant MADS... The MADS proteins APETALA3 (AP3), PISTILLATA (PI), SEPALLATA1 (SEP1), SEP2, SEP3, AGAMOUS, and APETALA1 are required for proper floral organ identity in Arabidopsis flowers. All of these floral MADS proteins conserve two domains: the MADS domain that mediates DNA binding and dimerization, and the K domain that mediates protein–protein interaction. The K domain is postulated to form a several amphipathic a-helices referred to as K1, K2, and K3. The K1 and K2 helicies are located entirely within the K domain while the K3 helix spans the K domain-C domain boundary. Here we report on our studies on the interactions of the B class MADS proteins AP3 and PI with the E class MADS proteins SEP1, SEP2, and SEP3. A comparative analysis of mutants in the K domain reveals that the subdomains mediating the PI/AP3 interaction are different from the subdomains mediating the PI/SEP3 (or PI/SEP1) interaction. The strong PI/SEP3 (or PI/SEP1) interaction requires K2, part of K3, and the interhelical region between K1 and K2. By contrast, K1, K2 and the region between K1 and K2 are important for strong AP3/PI interaction. Most of the K3 helix does not appear to be important for either the PI/AP3 or the Plant Molecular Biology Springer Journals

Defining subdomains of the K domain important for protein–protein interactions of plant MADS proteins

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Kluwer Academic Publishers
Copyright © 2004 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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