Plant Mol Biol (2017) 93:151–170 DOI 10.1007/s11103-016-0553-9 Deep analysis of wild Vitis flower transcriptome reveals unexplored genome regions associated with sex specification 1 1 2 3,4 Miguel Jesus Nunes Ramos · João Lucas Coito · Joana Fino · Jorge Cunha · 5 6 5 Helena Silva · Patrícia Gomes de Almeida · Maria Manuela Ribeiro Costa · 1 2 1 Sara Amâncio · Octávio S. Paulo · Margarida Rocheta Received: 29 March 2016 / Accepted: 15 October 2016 / Published online: 24 October 2016 © Springer Science+Business Media Dordrecht 2016 Abstract determines hermaphroditism in the domesticated subspecies Key message RNA-seq of Vitis during early stages of and male or female flowers in wild dioecious relatives ( Vitis bud development, in male, female and hermaphrodite vinifera ssp. sylvestris). Here, we describe a de novo assem- flowers, identified new l oci outside of annotated bly of the transcriptome of three flower developmental stages gene models, suggesting their involvement in sex from the three Vitis vinifera flower types. The validation of establishment. de novo assembly showed a correlation of 0.825. The main Abstract The molecular mechanisms responsible for flower goals of this work were the identification of V. v. sylvestris sex specification
Plant Molecular Biology – Springer Journals
Published: Oct 24, 2016
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