Daphne mosaic virus (DapMV), a new potyvirus from Daphne mezereum in the Czech Republic

Daphne mosaic virus (DapMV), a new potyvirus from Daphne mezereum in the Czech Republic Daphne shrubs with light green rings and mosaic on leaves contained flexuous filamentous virions (696 × 13 nm) and cylindrical inclusions typical of the subdivision III of Edwardson’s classification for inclusions induced by members of the family Potyviridae . Decoration tests using antisera to 67 potyviruses revealed distant serological relations among chilli veinal mottle virus, Colombian datura virus, papaya ringspot virus, tobacco vein mottling virus and yam mosaic virus. The 3′ terminal region of the virus genome was amplified by RT-PCR using primers specific for cloned and sequenced members of the family Potyviridae . The most similar sequences in the GenBank were those of isolates of wild potato mosaic virus (WPMV) and yam mild mosaic virus (YMMV), originating from Peru and Guadeloupe, respectively. The new sequence had 63.2% and 61.9% nucleotide identity to WPMV and YMMV in the coat protein gene. The results suggest that the Czech isolate from daphne should be regarded as a new member of the genus Potyvirus . The name daphne mosaic virus (DapMV) is suggested for this virus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Daphne mosaic virus (DapMV), a new potyvirus from Daphne mezereum in the Czech Republic

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Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer-Verlag/Wien
Subject
Biomedicine; Medical Microbiology; Infectious Diseases; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-005-0668-9
Publisher site
See Article on Publisher Site

Abstract

Daphne shrubs with light green rings and mosaic on leaves contained flexuous filamentous virions (696 × 13 nm) and cylindrical inclusions typical of the subdivision III of Edwardson’s classification for inclusions induced by members of the family Potyviridae . Decoration tests using antisera to 67 potyviruses revealed distant serological relations among chilli veinal mottle virus, Colombian datura virus, papaya ringspot virus, tobacco vein mottling virus and yam mosaic virus. The 3′ terminal region of the virus genome was amplified by RT-PCR using primers specific for cloned and sequenced members of the family Potyviridae . The most similar sequences in the GenBank were those of isolates of wild potato mosaic virus (WPMV) and yam mild mosaic virus (YMMV), originating from Peru and Guadeloupe, respectively. The new sequence had 63.2% and 61.9% nucleotide identity to WPMV and YMMV in the coat protein gene. The results suggest that the Czech isolate from daphne should be regarded as a new member of the genus Potyvirus . The name daphne mosaic virus (DapMV) is suggested for this virus.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2006

References

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