Cytotoxic Activity of Mellitin Expressed by Recombinant Vectors in Cells of Acholeplasma laidlawii and Mycoplasma hominis

Cytotoxic Activity of Mellitin Expressed by Recombinant Vectors in Cells of Acholeplasma... Recombinant plasmids containing the mellitin gene under the control of the lac and the tetM gene promoters were used for studying cytotoxic activity of mellitin in cells of mollicutes (mycoplasmas) and Escherichia coli. After transformation of Acholeplasma laidlawii andMycoplasma hominiscells with recombinant plasmid DNAs by electroporation, cell growth was suppressed. The expression of the mellitin gene in A. laidlawii andM. hominis cells was demonstrated using the reverse transcription polymerase chain reaction (RT–PCR). The possibility of using the mellitin gene in the recombinant vector as a potential antimycoplasmic gene-therapeutic agent with its selective expression in target cells is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Cytotoxic Activity of Mellitin Expressed by Recombinant Vectors in Cells of Acholeplasma laidlawii and Mycoplasma hominis

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Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1009066524968
Publisher site
See Article on Publisher Site

Abstract

Recombinant plasmids containing the mellitin gene under the control of the lac and the tetM gene promoters were used for studying cytotoxic activity of mellitin in cells of mollicutes (mycoplasmas) and Escherichia coli. After transformation of Acholeplasma laidlawii andMycoplasma hominiscells with recombinant plasmid DNAs by electroporation, cell growth was suppressed. The expression of the mellitin gene in A. laidlawii andM. hominis cells was demonstrated using the reverse transcription polymerase chain reaction (RT–PCR). The possibility of using the mellitin gene in the recombinant vector as a potential antimycoplasmic gene-therapeutic agent with its selective expression in target cells is discussed.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 16, 2004

References

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