Cytochalasin B Treatment of Apple (Malus pumila Mill.) Pollen Tubes Alters the Cytoplasmic Calcium Gradient and Causes Major Changes in the Cell Wall Components

Cytochalasin B Treatment of Apple (Malus pumila Mill.) Pollen Tubes Alters the Cytoplasmic... It is well established that the actin cytoskeleton is absolutely essential to pollen germination and tube growth. In this study we investigated the effects of cytochalasin B (CB), which affects actin polymerization by binding to the barbed end of actin filaments, on apple (Malus pumila Mill.) pollen tube growth. Results showed that CB altered the morphology of pollen tubes, which had a larger diameter than control tubes beside inhibiting pollen germination and tube growth. Meantime CB also caused an abnormal distribution of actin filaments in the shank of the treated pollen tubes. Fluo-3/AM labeling indicated that the gradient of cytosolic calcium ([Ca2+]c) in the pollen tube tip was abolished by exposure to CB, which induced a much stronger signal in the cytoplasm. Cellulose and callose distribution in the tube apex changed due to the CB treatment. Immunolabeling with different pectin and arabinogalactan protein (AGP) antibodies illustrated that CB induced an accumulation of pectins and AGPs in the tube cytoplasm and apex wall. The above results were further supported by Fourier-transform infrared (FTIR) analysis. The results suggest the disruption of actin can result in abnormal growth by disturbing the [Ca2+]c gradient and the distribution of cell wall components at the pollen tube apex. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Cytochalasin B Treatment of Apple (Malus pumila Mill.) Pollen Tubes Alters the Cytoplasmic Calcium Gradient and Causes Major Changes in the Cell Wall Components

10 pages
Read Article

Loading next page...
 
/lp/springer_journal/cytochalasin-b-treatment-of-apple-malus-pumila-mill-pollen-tubes-LZoy0KjG0T
Publisher
Springer Journals
Copyright
Copyright © 2018 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443718030111
Publisher site
See Article on Publisher Site

Abstract

It is well established that the actin cytoskeleton is absolutely essential to pollen germination and tube growth. In this study we investigated the effects of cytochalasin B (CB), which affects actin polymerization by binding to the barbed end of actin filaments, on apple (Malus pumila Mill.) pollen tube growth. Results showed that CB altered the morphology of pollen tubes, which had a larger diameter than control tubes beside inhibiting pollen germination and tube growth. Meantime CB also caused an abnormal distribution of actin filaments in the shank of the treated pollen tubes. Fluo-3/AM labeling indicated that the gradient of cytosolic calcium ([Ca2+]c) in the pollen tube tip was abolished by exposure to CB, which induced a much stronger signal in the cytoplasm. Cellulose and callose distribution in the tube apex changed due to the CB treatment. Immunolabeling with different pectin and arabinogalactan protein (AGP) antibodies illustrated that CB induced an accumulation of pectins and AGPs in the tube cytoplasm and apex wall. The above results were further supported by Fourier-transform infrared (FTIR) analysis. The results suggest the disruption of actin can result in abnormal growth by disturbing the [Ca2+]c gradient and the distribution of cell wall components at the pollen tube apex.

Journal

Russian Journal of Plant Physiology – Springer Journals

Published: Jun 1, 2018

References

  • Cell wall composition, biosynthesis and remodeling during pollen tube growth
    Mollet, J.C.; Leroux, C.; Dardelle, F.; Lehner, A.
  • Pollen specific expression of maize genes encoding actin depolymerizing factor-like proteins
    Lopez, I.; Anthony, R.G.; Maciver, S.K.; Jiang, C.J.; Khan, S.; Weeds, A.G.; Hussey, P.J.
  • Control of cell wall extensibility during pollen tube growth
    Hepler, P.K.; Rounds, C.M.; Winship, L.J.
  • The cytoskeleton in the pollen tube
    Fu, Y.
  • Organization and regulation of the actin cytoskeleton in the pollen tube
    Qu, X.L.; Jiang, Y.X.; Chang, M.; Liu, X.N.; Zhang, R.H.; Huang, S.J.
  • The actin cytoskeleton and signaling network during pollen tube tip growth
    Fu, Y.
  • Localized apical increases of cytosolic free calcium control pollen tube orientation
    Malhó, R.; Trewavas, A.J.
  • Calcium at the cell wall–cytoplast interface
    Hepler, P.K.; Winship, L.J.
  • Effects of cytochalasin and phalloidin on actin
    Cooper, J.A.
  • Latrunculin B has different effects on pollen germination and tube growth
    Gibbon, B.C.; Kovar, D.R.; Staiger, C.J.
  • Cytochalasin D inhibits actin polymerization and induces depolymerization of actin filaments formed during platelet shape change
    Casella, J.F.; Flanagan, M.D.; Lin, S.
  • Inhibition of intracellular pectin transport in pollen tubes by monensin, brefeldin A and cytochalasin D
    Geitmann, A.; Wojciechowicz, K.; Cresti, M.
  • Determination of secretory vesicle production rates by dictyosomes in pollen tubes of Trade scantia using cytochalasin D
    Picton, J.; Steer, M.W.
  • Cytochalasin B affects protein secretion in germinating Malus domestica Borkh.
    Speranza, A.; Calzont, G.L.
  • Addition of phenylboronic acid to Malus domestica pollen tubes alters calcium dynamics, disrupts actin filaments and affects cell wall architecture
    Fang, K.F.; Gao, S.; Zhang, W.W.; Xing, Y.; Cao, Q.Q.; Qin, L.
  • Pollen proteins bind to the C-terminal domain of Nicotiana alata pistil arabinogalactan proteins
    Lee, C.B.; Swatek, K.N.; McClure, B.
  • Cell wall assembly and intracellular trafficking in plant cells are directly affected by changes in the magnitude of gravitational acceleration
    Chebli, Y.; Pujo, L.; Shojaeifard, A.; Brouwer, I. v; Loon, J.J.; Geitmann, A.
  • Interaction between calcium and actin in guard cell and pollen signaling networks
    Chen, D.H.; Acharya, B.R.; Liu, W.; Zhang, W.
  • FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe
    Su, H.; Zhu, J.S.; Cai, C.; Pei, W.K.; Wang, J.J.; Dong, H.J.; Ren, H.Y.
  • Signaling in pollen tube growth: crosstalk, feedback, and missing links
    Guan, Y.F.; Guo, J.Z.; Li, H.; Yang, Z.B.
  • Actin is involved in pollen tube tropism through redefining the spatial targeting of secretory vesicles
    Bou Daher, F.; Geitmann, A.
  • Plant Developmental Biology—Biotechnological, Perspectives
    Shi, D.Q.; Yang, W.C.
  • Pollen tube growth oscillations and intracellular calcium levels are reversibly modulated by actin polymerization
    Cárdenas, L.; Lovy-Wheeler, A.; Kunkel, J.G.; Hepler, P.K.
  • Ca2+-permeable channels in the plasma membrane of Arabidopsis pollen are regulated by actin microfilaments
    Wang, Y.F.; Fan, L.M.; Zhang, W.Z.; Zhang, W.; Wu, W.H.
  • Cell wall, cytoskeleton, and cell expansion in higher plants
    Bashline, L.; Lei, L.; Li, S.D.; Gu, Y.
  • More than a leak sealant— the physical properties of callose in pollen tubes
    Parre, E.; Geitmann, A.
  • Molecular basis of Ca2+-induced gelation in alginates and pectins: the egg-box model revisited
    Braccini, I.; Perez, S.
  • The apical actin fringe contributes to localized cell wall deposition and polarized growth in the lily pollen tube
    Rounds, C.M.; Hepler, P.K.; Winship, L.J.
  • Stiffness of Ca2+–pectin gels: combined effects of degree and pattern of methylesterification for various Ca2+ concentrations
    Ngouémazong, D.E.; Jolie, R.P.; Cardinaels, R.; Fraeye, I. v; Loey, A.; Moldenaers, P.; Hendrickx, M.
  • Arabinogalactan-proteins: key regulators at the cell surface
    Ellis, M.; Egelund, J.; Schultz, C.J.; Bacic, A.

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial