Cyclic GMP-Binding Activity in Avena sativa Seedlings

Cyclic GMP-Binding Activity in Avena sativa Seedlings Binding of 3H-labeled cyclic GMP (3H-cGMP) to the structural components of subcellular fractions was studied in oat seedlings. The binding was found to depend on the conditions of incubation and illumination of growing plants. In the green seedlings, the binding activity was lower than in the etiolated seedlings. The highest binding was observed in soluble cytosolic fraction where two types of specific sites for cGMP binding (with high and low affinity to the cyclic mononucleotide) were detected. The binding activity was found to increase as a result of red light influence via phytochrome, as well as in the presence of calcium ions and calcium–calmodulin complex. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Cyclic GMP-Binding Activity in Avena sativa Seedlings

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1014801623322
Publisher site
See Article on Publisher Site

Abstract

Binding of 3H-labeled cyclic GMP (3H-cGMP) to the structural components of subcellular fractions was studied in oat seedlings. The binding was found to depend on the conditions of incubation and illumination of growing plants. In the green seedlings, the binding activity was lower than in the etiolated seedlings. The highest binding was observed in soluble cytosolic fraction where two types of specific sites for cGMP binding (with high and low affinity to the cyclic mononucleotide) were detected. The binding activity was found to increase as a result of red light influence via phytochrome, as well as in the presence of calcium ions and calcium–calmodulin complex.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 13, 2004

References

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