Cultivation, characterization, and properties of Chlorella vulgaris microalgae with different lipid contents and effect on fast pyrolysis oil composition

Cultivation, characterization, and properties of Chlorella vulgaris microalgae with different... A systematic study of the effect of nitrogen levels in the cultivation medium of Chlorella vulgaris microalgae grown in photobioreactor (PBR) on biomass productivity, biochemical and elemental composition, fatty acid profile, heating value (HHV), and composition of the algae-derived fast pyrolysis (bio-oil) is presented in this work. A relatively high biomass productivity and cell concentration (1.5 g of dry biomass per liter of cultivation medium and 120 × 106 cells/ml, respectively) were achieved after 30 h of cultivation under N-rich medium. On the other hand, the highest lipid content (ca. 36 wt.% on dry biomass) was obtained under N-depletion cultivation conditions. The medium and low N levels favored also the increased concentration of the saturated and mono-unsaturated C16:0 and C18:1(n-9) fatty acids (FA) in the lipid/oil fraction, thus providing a raw lipid feedstock that can be more efficiently converted to high-quality biodiesel or green diesel (via hydrotreatment). In terms of overall lipid productivity, taking in consideration both the biomass concentration in the medium and the content of lipids on dry biomass, the most effective system was the N-rich one. The thermal (non-catalytic) pyrolysis of Chlorella vulgaris microalgae produced a highly complex bio-oil composition, including fatty acids, phenolics, ethers, ketones, etc., as well as aromatics, alkanes, and nitrogen compounds (pyrroles and amides), originating from the lipid, protein, and carbohydrate fractions of the microalgae. However, the catalytic fast pyrolysis using a highly acidic ZSM-5 zeolite, afforded a bio-oil enriched in mono-aromatics (BTX), reducing at the same time significantly oxygenated compounds such as phenolics, acids, ethers, and ketones. These effects were even more pronounced in the catalytic fast pyrolysis of Chlorella vulgaris residual biomass (after extraction of lipids), thus showing for the first time the potential of transforming this low value by-product towards high added value platform chemicals. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Science and Pollution Research Springer Journals

Cultivation, characterization, and properties of Chlorella vulgaris microalgae with different lipid contents and effect on fast pyrolysis oil composition

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Environment; Environment, general; Environmental Chemistry; Ecotoxicology; Environmental Health; Atmospheric Protection/Air Quality Control/Air Pollution; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution
ISSN
0944-1344
eISSN
1614-7499
D.O.I.
10.1007/s11356-018-2368-5
Publisher site
See Article on Publisher Site

Abstract

A systematic study of the effect of nitrogen levels in the cultivation medium of Chlorella vulgaris microalgae grown in photobioreactor (PBR) on biomass productivity, biochemical and elemental composition, fatty acid profile, heating value (HHV), and composition of the algae-derived fast pyrolysis (bio-oil) is presented in this work. A relatively high biomass productivity and cell concentration (1.5 g of dry biomass per liter of cultivation medium and 120 × 106 cells/ml, respectively) were achieved after 30 h of cultivation under N-rich medium. On the other hand, the highest lipid content (ca. 36 wt.% on dry biomass) was obtained under N-depletion cultivation conditions. The medium and low N levels favored also the increased concentration of the saturated and mono-unsaturated C16:0 and C18:1(n-9) fatty acids (FA) in the lipid/oil fraction, thus providing a raw lipid feedstock that can be more efficiently converted to high-quality biodiesel or green diesel (via hydrotreatment). In terms of overall lipid productivity, taking in consideration both the biomass concentration in the medium and the content of lipids on dry biomass, the most effective system was the N-rich one. The thermal (non-catalytic) pyrolysis of Chlorella vulgaris microalgae produced a highly complex bio-oil composition, including fatty acids, phenolics, ethers, ketones, etc., as well as aromatics, alkanes, and nitrogen compounds (pyrroles and amides), originating from the lipid, protein, and carbohydrate fractions of the microalgae. However, the catalytic fast pyrolysis using a highly acidic ZSM-5 zeolite, afforded a bio-oil enriched in mono-aromatics (BTX), reducing at the same time significantly oxygenated compounds such as phenolics, acids, ethers, and ketones. These effects were even more pronounced in the catalytic fast pyrolysis of Chlorella vulgaris residual biomass (after extraction of lipids), thus showing for the first time the potential of transforming this low value by-product towards high added value platform chemicals.

Journal

Environmental Science and Pollution Research – Springer Journals

Published: Jun 1, 2018

References

  • Catalytic pyrolysis of microalgae to high-quality liquid bio-fuels
    Babich, IV; Hulst, M; Lefferts, L; Moulijn, JA; O’Connor, P; Seshan, K
  • Regulation of inorganic carbon acquisition by phosphorus limitation in the green alga Chlorella emersonii
    Beardall, J; Roberts, S; Raven, JA
  • Micro-algae as a source of protein
    Becker, EW
  • Catalytic pyrolysis-GC/MS of Spirulina: evaluation of a highly proteinaceous biomass source for production of fuels and chemicals
    Chagas, BME; Dorado, C; Serapiglia, MJ; Mullen, CA; Boateng, AA; Melo, MAF; Ataíde, CH
  • Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review
    Chen, C-Y; Yeh, K-L; Aisyah, R; Lee, D-J; Chang, J-S
  • Engineering strategies for the enhanced photo-H2 production using effluents of dark fermentation processes as substrate
    Chen, C-Y; Yeh, K-L; Lo, Y-C; Wang, H-M; Chang, J-S
  • Flow cytometry pulse width data enables rapid and sensitive estimation of biomass dry weight in the microalgae Chlamydomonas reinhardtii and Chlorella vulgaris
    Chioccioli, M; Hankamer, B; Ross, IL
  • Biodiesel from microalgae
    Chisti, Y
  • Production of FAMEs from several microalgal lipidic extracts and direct transesterification of the Chlorella pyrenoidosa
    D’Oca, MGM; Viêgas, CV; Lemões, JS; Miyasaki, EK; Morón-Villarreyes, JA; Primel, EG; Abreu, PC
  • The need to implement an efficient biomass fractionation and full utilization based on the concept of “biorefinery” for a viable economic utilization of microalgae
    Dibenedetto, A; Colucci, A; Aresta, M
  • Production of aromatic hydrocarbons by catalytic pyrolysis of microalgae with zeolites: catalyst screening in a pyroprobe
    Du, Z; Ma, X; Li, Y; Chen, P; Liu, Y; Lin, X; Lei, H; Ruan, R
  • Cascading microalgae biorefinery: fast pyrolysis of Dunaliella tertiolecta lipid extracted-residue
    Francavilla, M; Kamaterou, P; Intini, S; Monteleone, M; Zabaniotou, A
  • On the effectiveness of tailored mesoporous MFI zeolites for biomass catalytic fast pyrolysis
    Gamliel, DP; Cho, HJ; Fan, W; Valla, JA
  • Response of the photosynthetic apparatus of Phaeodactylum tricornutum (Bacillariophyceae) to nitrate, phosphate, or iron starvation1
    Geider, RJ; Roche, J; Greene, RM; Olaizola, M
  • Proximate biochemical composition and caloric content calculated from elemental CHN analysis: a stoichiometric concept
    Gnaiger, E; Bitterlich, G
  • Dependency of microalgal production on biomass and the relationship to yield and bioreactor scale-up for biofuels: a statistical analysis of 60+ years of algal bioreactor data
    Granata, T
  • Nitrogen limitation in Isochrysis galbana (Haptophyceae). I. Photosynthetic energy conversion and growth efficiencies1
    Herrig, R; Falkowski, PG
  • Perspectives on microalgal CO2-emission mitigation systems—a review
    Ho, S-H; Chen, C-Y; Lee, D-J; Chang, J-S
  • Characterization of photosynthetic carbon dioxide fixation ability of indigenous Scenedesmus obliquus isolates
    Ho, S-H; Chen, C-Y; Yeh, K-L; Chen, W-M; Lin, C-Y; Chang, J-S
  • Response of growth and fatty acid compositions of Nannochloropsis sp. to environmental factors under elevated CO2 concentration
    Hu, H; Gao, K
  • The effect of nitrogen limitation on the physiology and metabolism of chlorella vulgaris var L3
    Ikaran, Z; Suárez-Alvarez, S; Urreta, I; Castañón, S
  • Increase in Chlorella strains calorific values when grown in low nitrogen medium
    Illman, AM; Scragg, AH; Shales, SW
  • The feasibility of industrial production of Spirulina (Arthrospira) in southern Spain
    Jiménez, C; Cossı́o, BR; Labella, D; Xavier Niell, F
  • A critical evaluation of CO2 supplementation to algal systems by direct injection
    Langley, NM; Harrison, STL; Hille, RP
  • Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans
    Li, Y; Horsman, M; Wang, B; Wu, N; Lan, CQ
  • The enhanced lipid accumulation in oleaginous microalgae by the potential continuous nitrogen-limitation (CNL) strategy
    Liu, T; Li, Y; Liu, F; Wang, C
  • Cultivation of Chlorella sp. using raw dairy wastewater for nutrient removal and biodiesel production: characteristics comparison of indoor bench-scale and outdoor pilot-scale cultures
    Lu, W; Wang, Z; Wang, X; Yuan, Z
  • Algal biofuels: the eternal promise?
    Luque, R
  • Microalgae for biodiesel production and other applications: a review
    Mata, TM; Martins, AA; Caetano, NS
  • High yield bio-oil production from fast pyrolysis by metabolic controlling of Chlorella protothecoides
    Miao, X; Wu, Q
  • Screening acidic zeolites for catalytic fast pyrolysis of biomass and its components
    Mihalcik, DJ; Mullen, CA; Boateng, AA
  • A review on the assessment of stress conditions for simultaneous production of microalgal lipids and carotenoids
    Minhas, AK; Hodgson, P; Barrow, CJ; Adholeya, A
  • Production of γ-linolenic acid from the marine green alga Chlorella sp. NKG 042401
    Miura, Y; Sode, K; Nakamura, N; Matsunaga, N; Matsunaga, T
  • Heterotrophic cultivation of microalgae: production of metabolites of commercial interest
    Morales-Sánchez, D; Martinez-Rodriguez, OA; Martinez, A
  • Microalgae as substrates for fermentative biogas production in a combined biorefinery concept
    Mussgnug, JH; Klassen, V; Schlüter, A; Kruse, O
  • Impact of nitrogen limitation on biomass, photosynthesis, and lipid accumulation in Chlorella sorokiniana
    Negi, S; Barry, AN; Friedland, N; Sudasinghe, N; Subramanian, S; Pieris, S; Holguin, FO; Dungan, B; Schaub, T; Sayre, R
  • Trichosarcina polymorpha gen. et Sp Nov
    Nichols, HW; Bold, HC
  • Evaluation of methane production and macronutrient degradation in the anaerobic co-digestion of algae biomass residue and lipid waste
    Park, S; Li, Y
  • Microalgae Chlorella as a potential bio-energy feedstock
    Phukan, MM; Chutia, RS; Konwar, BK; Kataki, R
  • Life cycle assessment of green diesel production from microalgae
    Pragya, N; Pandey, KK
  • Production of lipids in 10 strains of Chlorella and Parachlorella, and enhanced lipid productivity in Chlorella vulgaris
    Přibyl, P; Cepák, V; Zachleder, V
  • Influence of fatty acid composition of raw materials on biodiesel properties
    Ramos, MJ; Fernández, CM; Casas, A; Rodríguez, L; Pérez, Á
  • Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor
    Rodolfi, L; Chini Zittelli, G; Bassi, N; Padovani, G; Biondi, N; Bonini, G; Tredici, MR
  • Environmental control of glycerolipid metabolism in microalgae: commercial implications and future research directions
    Roessler, PG
  • Stress enhances poly-unsaturation rich lipid accumulation in Chlorella sp. and Chlamydomonas sp
    Shekh, AY; Shrivastava, P; Krishnamurthi, K; Mudliar, SN; Devi, SS; Kanade, GS; Chakrabarti, T
  • A viable technology to generate third-generation biofuel
    Singh, A; Olsen, SI; Nigam, PS
  • Trends and novel strategies for enhancing lipid accumulation and quality in microalgae
    Singh, P; Kumari, S; Guldhe, A; Misra, R; Rawat, I; Bux, F
  • A study of lignocellulosic biomass pyrolysis via the pyrolysis of cellulose, hemicellulose and lignin
    Stefanidis, SD; Kalogiannis, KG; Iliopoulou, EF; Michailof, CM; Pilavachi, PA; Lappas, AA
  • Catalytic upgrading of lignocellulosic biomass pyrolysis vapours: effect of hydrothermal pre-treatment of biomass
    Stephanidis, S; Nitsos, C; Kalogiannis, K; Iliopoulou, EF; Lappas, AA; Triantafyllidis, KS
  • Microalgae (Nannochloropsis Salina) biomass to lactic acid and lipid
    Talukder, MMR; Das, P; Wu, JC
  • Catalytic pyrolysis of green algae for hydrocarbon production using H+ZSM-5 catalyst
    Thangalazhy-Gopakumar, S; Adhikari, S; Chattanathan, SA; Gupta, RB
  • Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa
    Waghmare, AG; Salve, MK; LeBlanc, JG; Arya, SS
  • Effects of using light-emitting diodes on the cultivation of Spirulina platensis
    Wang, C-Y; Fu, C-C; Liu, Y-C
  • Catalytic pyrolysis of microalgae for production of aromatics and ammonia
    Wang, K; Brown, RC
  • Fast pyrolysis of microalgae remnants in a fluidized bed reactor for bio-oil and biochar production
    Wang, K; Brown, RC; Homsy, S; Martinez, L; Sidhu, SS
  • Catalytic pyrolysis of individual components of lignocellulosic biomass
    Wang, K; Kim, KH; Brown, RC
  • Catalytic hydroprocessing of microalgae-derived biofuels: a review
    Yang, C; Li, R; Cui, C; Liu, S; Qiu, Q; Ding, Y; Wu, Y; Zhang, B
  • Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalgae Chlorella vulgaris ESP-31
    Yeh, K-L; Chang, J-S
  • Effect of light quality on production of extracellular polysaccharides and growth rate of Porphyridium cruentum
    You, T; Barnett, SM
  • Catalytic deoxygenation of microalgae oil to green hydrocarbons
    Zhao, C; Bruck, T; Lercher, JA
  • Influence of nitrogen deficiency on biochemical composition of the green alga Botryococcus
    Zhila, NO; Kalacheva, GS; Volova, TG

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