1022-7954/03/3912- $25.00 © 2003
Russian Journal of Genetics, Vol. 39, No. 12, 2003, pp. 1465–1466. Translated from Genetika, Vol. 39, No. 12, 2003, pp. 1723–1724.
Original Russian Text Copyright © 2003 by Goncharov, Bannikova, Peltek.
Cultivar identiﬁcation in crop species is a long-
standing problem . In common wheat, cultivar iden-
tiﬁcation was based on the analysis of morphological
traits for a long period. More recently, speciﬁc methods
were developed and storage proteins of the endosperm
came to be employed in cultivar identiﬁcation [2, 3]. As
a result of these studies, which were carried out in Rus-
sia and other countries, catalogs of standard protein
spectra of common wheat cultivars were published to
expedite cultivar identiﬁcation (examination) [4, 6].
Monosomic analysis was widely employed in genetic
studies of common wheat. A considerable number of
monosomic series were produced for this purpose, and
one-ﬁfth of these were constructed in Russia . Two
Russian series most commonly involved in such studies
were based on Russian commercial cultivar Sara-
tovskaya 29 and on a Swedish cultivar . Since the
source of the latter was unknown, the originators of the
corresponding monosomic series indicated it in a differ-
ent way, namely: Diamant , Diamant 2 , Diamant 1
[11, 12], and, again, Diamant . Cultivar identiﬁcation
of this monosomic series was difﬁcult, because cultivar
Diamant was not preserved in the collection of the All-
Russian Institute of the Plant Industry (St. Petersburg).
Recently, the cultivar was found in the doublet collection
of the Siberian Institute of the Plant Industry and Breed-
ing (Krasnoobsk, Novosibirsk Oblast), which harbors
old, local, and commercial cultivars of Siberia. The cul-
tivar was kindly provided by A.Ya. Sotnik.
This work was aimed at cultivar identiﬁcation of the
monosomic series by alcohol-soluble storage proteins
We used cultivars Diamant (k-59019), Diamant 2
(k-38691), and Saratovskaya 29. Gliadins were assayed
as described by Peltek
. Protein content per
grain was established and compared for different culti-
vars by Student’s pairwise
test for small samples
according to the standard methods.
Spectra of storage proteins were established for cul-
tivars Diamant, Diamant 2, and for a Swedish cultivar,
which was maintained in the Laboratory of Plant Chro-
mosomal Engineering, Institute of Cytology and
Genetics, and was used to construct the monosomic
series (ﬁgure). It is seen that the spectrum of storage
proteins in euploid plants was much the same in culti-
vars Diamant and Diamant 2, being to an extent poly-
morphic in the former.
Since a high protein content is characteristic of
Swedish cultivars, we compared the cultivars with
respect to this parameter. By Kieldal method, grain pro-
of a Monosomic Common Wheat Series
N. P. Goncharov, S. V. Bannikova, and S. E. Peltek
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia;
fax: (3832)33-12-78; e-mail: firstname.lastname@example.org
Received March 24, 2003
—Storage proteins (gliadins) were employed in cultivar identiﬁcation of a monosomic series pro-
duced on the basis of a Swedish cultivar. The cultivar was identiﬁed as Diamant 2.
Electrophoresis of gliadins of common wheat cultivars
) Diamant and (
) Diamant 2 and (
) an eup-
loid plant of the Swedish cultivar, which was used to obtain
the monosomic series.