Cross-protection of Lactococcus lactis -displayed HA2 subunit against homologous and heterologous influenza A viruses in mice

Cross-protection of Lactococcus lactis -displayed HA2 subunit against homologous and heterologous... Current influenza vaccines provide strain-specific protection against homologous subtypes and need to be updated annually. Therefore, it is essential to develop a universal vaccine that would induce broadly cross-protective immunity against homologous and heterologous influenza A viruses. The highly conserved HA2 subunit is a promising candidate for developing a universal influenza vaccine. Here, we hypothesized that the HA2 subunit could be displayed on the surface of Lactococcus lactis ( L. lactis ), using Spax as an anchor protein ( L. lactis /pNZ8008-Spax-HA2) and that L. lactis /pNZ8008-Spax-HA2 would have immunogenicity by oral administration without the use of adjuvant in the mouse model. To address this hypothesis, we show that oral vaccination of mice with L. lactis /pNZ8008-Spax-HA2 elicited significant humoral and mucosal immune responses. Importantly, L. lactis /pNZ8008-Spax-HA2 provided 100 % protection against homologous H5N1 or heterologous H1N1 virus challenge. These results suggest that an HA2 subunit presented on the surface of L. lactis is an effective universal vaccine candidate against influenza A viruses in the poultry industry and in humans. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Cross-protection of Lactococcus lactis -displayed HA2 subunit against homologous and heterologous influenza A viruses in mice

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2587-8
Publisher site
See Article on Publisher Site

References

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  • Intranasal immunization with live recombinant Lactococcus lactis combined with heat-labile toxin B subunit protects chickens from highly pathogenic avian influenza H5N1 virus
    Lei, H; Peng, X; Shu, H; Zhao, D
  • Intranasal immunization of recombinant Lactococcus lactis induces protection against H5N1 virus in ferrets
    Lei, H; Peng, X; Ouyang, J; Zhao, D; Jiao, H; Shu, H; Ge, X
  • Surface expression of Helicobacter pylori urease subunit B gene E fragment on Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A
    Song, D; Gu, Q
  • The highly conserved HA2 protein of the influenza A virus induces a cross protective immune response
    Lee, JS; Chowdhury, MY; Moon, HJ; Choi, YK; Talactac, MR; Kim, JH; Park, ME; Son, HY; Shin, KS; Kim, CJ

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