Cross-protection of Lactococcus lactis -displayed HA2 subunit against homologous and heterologous influenza A viruses in mice

Cross-protection of Lactococcus lactis -displayed HA2 subunit against homologous and heterologous... Current influenza vaccines provide strain-specific protection against homologous subtypes and need to be updated annually. Therefore, it is essential to develop a universal vaccine that would induce broadly cross-protective immunity against homologous and heterologous influenza A viruses. The highly conserved HA2 subunit is a promising candidate for developing a universal influenza vaccine. Here, we hypothesized that the HA2 subunit could be displayed on the surface of Lactococcus lactis ( L. lactis ), using Spax as an anchor protein ( L. lactis /pNZ8008-Spax-HA2) and that L. lactis /pNZ8008-Spax-HA2 would have immunogenicity by oral administration without the use of adjuvant in the mouse model. To address this hypothesis, we show that oral vaccination of mice with L. lactis /pNZ8008-Spax-HA2 elicited significant humoral and mucosal immune responses. Importantly, L. lactis /pNZ8008-Spax-HA2 provided 100 % protection against homologous H5N1 or heterologous H1N1 virus challenge. These results suggest that an HA2 subunit presented on the surface of L. lactis is an effective universal vaccine candidate against influenza A viruses in the poultry industry and in humans. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Cross-protection of Lactococcus lactis -displayed HA2 subunit against homologous and heterologous influenza A viruses in mice

Loading next page...
 
/lp/springer_journal/cross-protection-of-lactococcus-lactis-displayed-ha2-subunit-against-DK2vtNqckv
Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2587-8
Publisher site
See Article on Publisher Site

Abstract

Current influenza vaccines provide strain-specific protection against homologous subtypes and need to be updated annually. Therefore, it is essential to develop a universal vaccine that would induce broadly cross-protective immunity against homologous and heterologous influenza A viruses. The highly conserved HA2 subunit is a promising candidate for developing a universal influenza vaccine. Here, we hypothesized that the HA2 subunit could be displayed on the surface of Lactococcus lactis ( L. lactis ), using Spax as an anchor protein ( L. lactis /pNZ8008-Spax-HA2) and that L. lactis /pNZ8008-Spax-HA2 would have immunogenicity by oral administration without the use of adjuvant in the mouse model. To address this hypothesis, we show that oral vaccination of mice with L. lactis /pNZ8008-Spax-HA2 elicited significant humoral and mucosal immune responses. Importantly, L. lactis /pNZ8008-Spax-HA2 provided 100 % protection against homologous H5N1 or heterologous H1N1 virus challenge. These results suggest that an HA2 subunit presented on the surface of L. lactis is an effective universal vaccine candidate against influenza A viruses in the poultry industry and in humans.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2015

References

  • Rapid cloning of high-affinity human monoclonal antibodies against influenza virus
    Wrammert, J; Smith, K; Miller, J; Langley, WA; Kokko, K; Larsen, C; Zheng, NY; Mays, I; Garman, L; Helms, C; James, J; Air, GM; Capra, JD; Ahmed, R; Wilson, PC
  • Intranasal immunization with live recombinant Lactococcus lactis combined with heat-labile toxin B subunit protects chickens from highly pathogenic avian influenza H5N1 virus
    Lei, H; Peng, X; Shu, H; Zhao, D
  • Intranasal immunization of recombinant Lactococcus lactis induces protection against H5N1 virus in ferrets
    Lei, H; Peng, X; Ouyang, J; Zhao, D; Jiao, H; Shu, H; Ge, X
  • Surface expression of Helicobacter pylori urease subunit B gene E fragment on Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A
    Song, D; Gu, Q
  • The highly conserved HA2 protein of the influenza A virus induces a cross protective immune response
    Lee, JS; Chowdhury, MY; Moon, HJ; Choi, YK; Talactac, MR; Kim, JH; Park, ME; Son, HY; Shin, KS; Kim, CJ

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off