Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth

Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus... Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. It is well established that the ARV sigma-C protein induces apoptosis in host cells. However, the underlying molecular mechanism of this induction is still unclear. We report here the identification of eukaryotic elongation factor 1 alpha 1 (EEF1A1) as the interacting partner of σC. We found that σC-induced apoptosis in DF-1 cells could be completely abolished by knockdown of EEF1A1 by siRNA. Furthermore, knockdown of EEF1A1 markedly reduced ARV-induced apoptosis associated with decreased caspase-9 and -3 activation and cytochrome C release, leading to increased ARV growth in host cells. Thus, EEF1A1 plays a critical role in σC-induced apoptosis and inhibition of viral growth. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2403-5
Publisher site
See Article on Publisher Site

Abstract

Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. It is well established that the ARV sigma-C protein induces apoptosis in host cells. However, the underlying molecular mechanism of this induction is still unclear. We report here the identification of eukaryotic elongation factor 1 alpha 1 (EEF1A1) as the interacting partner of σC. We found that σC-induced apoptosis in DF-1 cells could be completely abolished by knockdown of EEF1A1 by siRNA. Furthermore, knockdown of EEF1A1 markedly reduced ARV-induced apoptosis associated with decreased caspase-9 and -3 activation and cytochrome C release, leading to increased ARV growth in host cells. Thus, EEF1A1 plays a critical role in σC-induced apoptosis and inhibition of viral growth.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2015

References

  • Avian reovirus: structure and biology
    Benavente, J; Martinez-Costas, J
  • Reovirus-induced apoptosis: a minireview
    Clarke, P; Tyler, KL
  • Retardation of cell growth by avian reovirus p17 through the activation of p53 pathway
    Liu, HJ; Lin, PY; Lee, JW; Hsu, HY; Shih, WL
  • Apoptosis induction by avian reovirus through p53 and mitochondria-mediated pathway
    Chulu, JL; Lee, LH; Lee, YC
  • Avian reovirus S1133-induced DNA damage signaling and subsequent apoptosis in cultured cells and in chickens
    Lin, PY; Liu, HJ; Chang, CD
  • Avian reovirus activates a novel proapoptotic signal by linking Src to p53
    Ping-Yuan, L; Hung-Jen, L; Meng-Jiun, L

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