Plant Mol Biol (2017) 94:349–360 DOI 10.1007/s11103-017-0599-3 CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.) 1 1 1,2 Madhusudhana R. Janga · LeAnne M. Campbell · Keerti S. Rathore Received: 4 December 2016 / Accepted: 23 February 2017 / Published online: 3 March 2017 © Springer Science+Business Media Dordrecht 2017 Abstract The clustered, regularly interspaced, short pal- The results provide useful information that will help us tar- indromic repeats (CRISPR)/CRISPR associated (Cas)9 get important native genes in the cotton plant in future. protein system has emerged as a simple and efficient tool for genome editing in eukaryotic cells. It has been shown Keywords CRISPR/Cas9 · Gene knockout · GFP · to be functional in several crop species, yet there are no Gossypium hirsutum · sgRNAs · Targeted mutagenesis reports on the application of this or any other genome edit- ing technologies in the cotton plant. Cotton is an impor- tant crop that is grown mainly for its fiber, but its seed also Introduction serves as a useful source of edible oil and feed protein. Most of the commercially-grown cotton is tetraploid, thus Among the various genome-editing technologies, the making it much more difficult to target both sets of home- CRISPR/Cas9 system from Streptococcus pyogenes has ologous alleles. Therefore, in
Plant Molecular Biology – Springer Journals
Published: Mar 3, 2017
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