CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.)

CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.) Plant Mol Biol (2017) 94:349–360 DOI 10.1007/s11103-017-0599-3 CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.) 1 1 1,2 Madhusudhana R. Janga  · LeAnne M. Campbell  · Keerti S. Rathore   Received: 4 December 2016 / Accepted: 23 February 2017 / Published online: 3 March 2017 © Springer Science+Business Media Dordrecht 2017 Abstract The clustered, regularly interspaced, short pal- The results provide useful information that will help us tar- indromic repeats (CRISPR)/CRISPR associated (Cas)9 get important native genes in the cotton plant in future. protein system has emerged as a simple and efficient tool for genome editing in eukaryotic cells. It has been shown Keywords CRISPR/Cas9 · Gene knockout · GFP · to be functional in several crop species, yet there are no Gossypium hirsutum · sgRNAs · Targeted mutagenesis reports on the application of this or any other genome edit- ing technologies in the cotton plant. Cotton is an impor- tant crop that is grown mainly for its fiber, but its seed also Introduction serves as a useful source of edible oil and feed protein. Most of the commercially-grown cotton is tetraploid, thus Among the various genome-editing technologies, the making it much more difficult to target both sets of home- CRISPR/Cas9 system from Streptococcus pyogenes has ologous alleles. Therefore, in Plant Molecular Biology Springer Journals

CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.)

Loading next page...
Springer Netherlands
Copyright © 2017 by Springer Science+Business Media Dordrecht
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial