CRISPR-Cas Technology in Plant Science

CRISPR-Cas Technology in Plant Science Potato Research https://doi.org/10.1007/s11540-018-9387-y Peter M. Rogowsky Received: 4 January 2018 /Accepted: 4 April 2018 European Association for Potato Research 2018 Abstract CRISPR-Cas technology has raised considerable interest among plant scientists, both in basic science and in plant breeding. Presently, the generation of random mutations at a predetermined site of the genome is well mastered, just like the targeted insertion of transgenes, although both remain restricted to species or geno- types amenable for plant transformation. On the other hand, true genome editing, i.e. the deliberate replacement of one or several nucleotides of the genome in a predetermined fashion, is limited to some rather particular examples that generally concern genes allowing positive selection, for example tolerance to herbicides. Therefore, further technological developments are necessary to fully exploit the potential of genome editing in enlarging the gene pool beyond the natural variability available in a given species. In principle, the technology can be applied to any quality related, agronomical or ecological trait, under the condition of upstream knowledge on the genes to be targeted and the precise modifications necessary to improve alleles. Published proof of concepts concern a wide range of agronomical traits, the most frequent being disease resistance, herbicide tolerance and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Potato Research Springer Journals

CRISPR-Cas Technology in Plant Science

Potato Research , Volume OnlineFirst – Jun 1, 2018
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Publisher
Springer Netherlands
Copyright
Copyright © 2018 by European Association for Potato Research
Subject
Life Sciences; Plant Sciences; Agriculture; Plant Genetics and Genomics; Plant Systematics/Taxonomy/Biogeography
ISSN
0014-3065
eISSN
1871-4528
D.O.I.
10.1007/s11540-018-9387-y
Publisher site
See Article on Publisher Site

Abstract

Potato Research https://doi.org/10.1007/s11540-018-9387-y Peter M. Rogowsky Received: 4 January 2018 /Accepted: 4 April 2018 European Association for Potato Research 2018 Abstract CRISPR-Cas technology has raised considerable interest among plant scientists, both in basic science and in plant breeding. Presently, the generation of random mutations at a predetermined site of the genome is well mastered, just like the targeted insertion of transgenes, although both remain restricted to species or geno- types amenable for plant transformation. On the other hand, true genome editing, i.e. the deliberate replacement of one or several nucleotides of the genome in a predetermined fashion, is limited to some rather particular examples that generally concern genes allowing positive selection, for example tolerance to herbicides. Therefore, further technological developments are necessary to fully exploit the potential of genome editing in enlarging the gene pool beyond the natural variability available in a given species. In principle, the technology can be applied to any quality related, agronomical or ecological trait, under the condition of upstream knowledge on the genes to be targeted and the precise modifications necessary to improve alleles. Published proof of concepts concern a wide range of agronomical traits, the most frequent being disease resistance, herbicide tolerance and

Journal

Potato ResearchSpringer Journals

Published: Jun 1, 2018

References

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