ISSN 10227954, Russian Journal of Genetics, 2015, Vol. 51, No. 8, pp. 752–756. © Pleiades Publishing, Inc., 2015.
Original Russian Text © E.V. Dubina, Zh.M. Mukhina, E.M. Kharitonov, V.N. Shilovskiy, E.S. Kharchenko, L.V. Esaulova, N.N. Korkina, E.P. Maximenko, I.B. Nikitina, 2015,
published in Genetika, 2015, Vol. 51, No. 8, pp. 881–886.
One of the major limiting biotic stress factors for
rice is blast disease (the pathogen is imperfect fungus
The World Institute for Mycology has registered
this disease in more than 80 countries. In recent years,
this disease was epiphytotic in all ricegrowing regions
of the world.
In 2013, the rice fields of Kuban (Krasnodar Krai)
suffered from epiphytoties. Blast disease affected more
than 20% of rice crops. Grain yield losses amounted to
100–104 thousand tons (from the data of the Ministry
of Agriculture and Processing Industry of the Krasno
dar Krai for 2013).
One of the most costeffective ways to combat this
disease is to create resistant varieties, which will simul
taneously reduce the use of fungicides and minimize
yield losses. The use of modern biotechnological
methods (DNAmarkers) facilitates the breeding work
in this direction and makes it possible to rapidly create
gene sources with introgression and pyramiding genes
for resistance to the disease.
The goal of our research is to create blast disease–
resistant varieties and lines of rice with molecular
DNA markers. Particularly noteworthy is the creation
of varieties carrying several pyramided (combined in a
single genotype) genes for resistance to the pathogen.
Lines and varieties with three genes for resistance are
more resistant to blast disease than lines with specific
Based on the goal set during the study, the following
tasks will be carried out.
(1) Implementation of a program for the introduc
tion and pyramiding of the blast disease–resistance
highyielding rice varieties.
(2) Assessment of the created breeding material for
the presence of the above genes in the genotype by
(3) Development of a system of multiplex PCR to
identify in the hybrid offspring several of the above
(4) Rating of the created selection material by a
complex of selectionarily important traits under the
conditions of vegetation and field experiments.
(5) Performance of a phytopathological test to
assess the established breeding material for resistance
to the Krasnodar population of the pathogen.
MATERIALS AND METHODS
Donors for the introduction of blast disease–resis
tance genes into the germplasm of the Russian rice
varieties Flagman and Snezhinka consisted of varieties
and lines of foreign selection: IR36 (donor of gene
), BL1 (donor of gene
), C101LAC (donor
), and C101A51 (
inary assessment of donor lines for sensitivity to local
populations of the blastdisease pathogen by inoculat
ing rice plants with the fungal culture showed the resis
tance of the tested lines. However, in the south of Rus
sia, these donor lines proved to be lateripening, with
a vegetation season of 140–155 days, and were charac
terized by low fertility. The local area of rice produc
tion allows the cultivation of varieties maturing in no
more than 125 days.
Creation of Blast DiseaseResistant Rice Varieties
with Modern DNAMarkers
E. V. Dubina
, Zh. M. Mukhina
, E. M. Kharitonov
, V. N. Shilovskiy
E. S. Kharchenko
, L. V. Esaulova
, N. N. Korkina
, E. P. Maximenko
, and I. B. Nikitina
AllRussia Rice Research Institute, pos. Belozernyi, Krasnodar Krai, 350921 Russia
Elite Seed Production Enterprise “Krasnoe”, pos. Risoopytnyi, Krasnodar Krai, 353810 Russia
Received July 29, 2014
—Based on modern technologies of molecular DNAmarkers, blast disease–resistance genes (
) were introgressed and pyramided into domestic rice varieties to give them long
term disease resistance. For that purpose, this case study uses SSRmarkers closely linked to these genes, as
well as intragenic markers of genes
. Multiplex PCR systems were created for simultaneous
identification of two resistance genes in the hybrid progeny for the following combinations: