Controlled synthesis of icosahedral gold nanocrystals, and their self-assembly with an ionic liquid for enhanced immunosensing of squamous cell carcinoma antigen

Controlled synthesis of icosahedral gold nanocrystals, and their self-assembly with an ionic... Icosahedral gold nanocrystals (AuNCs) were synthesized by using the ionic liquid (IL) 1-(10-bromodecyl)-3-methyl-imidazolium bromide as a morphology-controlling reagent. The novel IL 1,3-di-(3-mercaptopropyl)-imidazolium bromide was employed as a bifunctional monomer to integrate the AuNCs onto the surface of a glassy carbon electrode (GCE) modified with gold nanoparticles. After interaction with thioglycolic acid (TGA) and activation of the carboxy groups, the antibody against squamous cell carcinoma antigen was immobilized on the modified GCE. Residual active sites were blocked with bovine serum albumin. The new materials and the steps for immunosensor fabrication were fully characterized. The reduction in peak current (using hexacyanoferrate as an electrochemical probe) due to the recognition of SCCA is linearly related to the SCCA concentration in the 0.02 to 10 ng mL−1 concentration range. The detection limit is as low as 12.6 pg mL−1 (at an S/N ratio of 3). The assay was applied to the quantitation of SCCA in human serum samples. The accuracy was validated by comparison with an ELISA method. The two methods displayed excellent consistency. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

Controlled synthesis of icosahedral gold nanocrystals, and their self-assembly with an ionic liquid for enhanced immunosensing of squamous cell carcinoma antigen

Loading next page...
 
/lp/springer_journal/controlled-synthesis-of-icosahedral-gold-nanocrystals-and-their-self-0ekgJM417W
Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag GmbH Austria
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-017-2377-y
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial