We developed systems of in vitrotranscription comprising chromatin or nuclei from barley leaves of various ages and from Arabidopsis thalianaleaves. We isolated a protein with a mol wt of 67 kD from both plant species. In the presence of cytokinin, this protein enhanced the elongation of RNA synthesis in both in vitrosystems. The effect of 67-kD protein depended on the presence of a specific hormone. The effect of a hormone–receptor complex on transcription in the system containing chromatin and RNA polymerase I from barley leaves of various ages was consistent with the effect of cytokinin on transcription in the intact leaves of various ages. A combination of the 67-kD receptor protein from A. thalianaleaves and trans-zeatin enhanced transcription in the system from barley leaves, and reciprocally, the 67-kD protein from barley leaves induced a cytokinin-dependent transcription activation in the system from A. thalianaleaves. This evidence indicates the absence of species specificity of the cytokinin receptor. A cytokinin-dependent activation of protein kinase activity of the 67-kD protein from barley leaves was demonstrated.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 10, 2004
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