Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration

Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration Induction of pluripotent cells termed callus by auxin represents a typical cell fate change required for plant in vitro regeneration; however, the molecular control of auxin-induced callus formation is largely elusive. We previously identified four Arabidopsis auxin-inducible Lateral Organ Boundaries Domain (LBD) transcription factors that govern callus formation. Here, we report that Arabidopsis basic region/leucine zipper motif 59 (AtbZIP59) transcription factor forms complexes with LBDs to direct auxin-induced callus formation. We show that auxin stabilizes AtbZIP59 and enhances its interaction with LBD, and that disruption of AtbZIP59 dampens auxin-induced callus formation whereas overexpression of AtbZIP59 triggers autonomous callus formation. AtbZIP59–LBD16 directly targets a FAD-binding Berberine (FAD-BD) gene and promotes its transcription, which contributes to callus formation. These findings define the AtbZIP59–LBD complex as a critical regulator of auxin-induced cell fate change during callus formation, which provides a new insight into the molecular regulation of plant regeneration and possible developmental programs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Plants Springer Journals

Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration

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Publisher
Nature Publishing Group UK
Copyright
Copyright © 2017 by The Author(s)
Subject
Life Sciences; Life Sciences, general; Plant Sciences
eISSN
2055-0278
D.O.I.
10.1038/s41477-017-0095-4
Publisher site
See Article on Publisher Site

Abstract

Induction of pluripotent cells termed callus by auxin represents a typical cell fate change required for plant in vitro regeneration; however, the molecular control of auxin-induced callus formation is largely elusive. We previously identified four Arabidopsis auxin-inducible Lateral Organ Boundaries Domain (LBD) transcription factors that govern callus formation. Here, we report that Arabidopsis basic region/leucine zipper motif 59 (AtbZIP59) transcription factor forms complexes with LBDs to direct auxin-induced callus formation. We show that auxin stabilizes AtbZIP59 and enhances its interaction with LBD, and that disruption of AtbZIP59 dampens auxin-induced callus formation whereas overexpression of AtbZIP59 triggers autonomous callus formation. AtbZIP59–LBD16 directly targets a FAD-binding Berberine (FAD-BD) gene and promotes its transcription, which contributes to callus formation. These findings define the AtbZIP59–LBD complex as a critical regulator of auxin-induced cell fate change during callus formation, which provides a new insight into the molecular regulation of plant regeneration and possible developmental programs.

Journal

Nature PlantsSpringer Journals

Published: Jan 22, 2018

References

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