An approach to describe continuous partitioning of Cerrena unicolor laccase in a PEG 6000—phosphate aqueous two-phase system was proposed. The laccase was separated from crude supernatant of C. unicolor-submerged culture, and all the experiments were carried out in 25 °C and pH 7 conditions. Masses of both phases and their compositions at phase equilibrium, as well as laccase activity concentrations at different mixing points, were measured in batch experiments. An empirical short-cut method was developed which allows for calculation of mass and volume fractions of the phases, laccase concentration factors, and laccase recoveries. Theoretical predictions were verified by several experiments carried out in a special mixer-settler unit with automatic substrate feed and continuous collection of separated phases. Required concentration of the laccase was possible to achieve in a one-step extraction process in the mixer-settler unit. The predictions of the short-cut method were compared to the results of experimental measurements of phase compositions, phase volume fractions, concentration factors and enzymatic yields at steady-state operation of the extraction unit. The values of experimental results lay well within the 10% error range of the predicted values.
Chemical Papers – Springer Journals
Published: Nov 11, 2017
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.
All for just $49/month
Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.
Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.
It’s easy to organize your research with our built-in tools.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera