ISSN 1063-0740, Russian Journal of Marine Biology, 2008, Vol. 34, No. 1, pp. 64–72. © Pleiades Publishing, Ltd., 2008.
Original Russian Text © V.Ya. Kavun, 2008, published in Biologiya Morya.
Crustaceans are among the organisms that are fre-
quently used as indicators to assess the state of the envi-
ronment [22, 23]. The grass shrimp
one of the most common decapod crustaceans in the
community, dominating in terms of biomass
[8, 14]. This species occurs in large numbers at the
islands of the Lesser Kurilskaya Ridge.
been studied for more than 50 years at the coasts of the
Sakhalin and the Kuril Islands ; however, there is no
data on heavy metal levels in the grass shrimp from this
region. At the same time, information on the tissue trace
metal composition of grass shrimp is needed, as this
species is commercially important in some areas of the
Sea of Japan and the Sea of Okhotsk [2, 5, 7, 14]. The
present paper investigates heavy metal levels in the
hepatopancreas, muscle tissue, and carapace (organs
and tissue that are widely used in research of this kind)
of the grass shrimp
MATERIAL AND METHODS
The grass shrimp
is a proteran-
drous hermaphrodite; therefore, during the ﬁrst years
of life its sex organs function as testes, and after the
transition stage they act as an ovary. Thus, the follow-
ing sex groups can be distinguished in this shrimp spe-
cies—males (m), half-female specimens (hf), and
females (f), based on the shape of endopod of the ﬁrst
pair of abdominal limbs .
Samples were collected in the coastal waters of the
Lesser Kurilskaya Ridge near the islands of Tanﬁlíev
(stn. 1), Zelenyi (stn. 2), and Yuri (stn. 3) (Fig. 1). The
sampling was carried out using the standard shrimp
traps for Japanese produce. The upper ring diameter
was 75 cm, the lower ring diameter was 95 cm, the trap
height was 40 cm and the diameter of the inlet opening,
including the ring, was 5 cm.
The body length of grass shrimp was measured with
a ruler; the weight of each specimen was determined on
an electronic balance with an accuracy of up to 0.1 g.
The commercial length was measured from the poste-
rior edge of the eye hole to the end of the telson on the
dorsal side. This parameter is more stable than is the
total body length measured from the beginning of the
rostrum, which is usually broken off during sampling.
A total of 88 males, 74 half-female specimens, and
154 females were used in experiments.
Metal concentrations in the shrimp tissues were
determined after preliminary preparation of the sam-
ples. To this end, the tissue samples were dried at 85
to a constant weight and dissolved in concentrated
nitric acid. The concentration of metals was determined
by ﬂame atomic absorption spectrometry .
Statistical processing of results was performed
using the Statistica and Excel XP program package.
Figures 2–5 show the concentrations of metals (Fe,
Mn, Zn, Cu, Pb, Cd, and Ni) in the hepatopancreas, car-
apace, and muscle tissue of the grass shrimp
For convenience of representation, metal data
are given separately for each element.
Content of Microelements in the Grass Shrimp
(Decapoda: Pandalidae) from Coastal Waters of the Lesser
V. Ya. Kavun
Institute of Marine Biology, Far East Division, Russian Academy of Sciences, Vladivostok, 690041 Russia
Accepted May 17, 2007
—The concentrations of Fe, Zn, Cu, Cd, Mn, Pb, and Ni were determined in the hepatopancreas, mus-
cle tissue, and carapace of the grass shrimp
from the coastal waters of the Lesser Kurilskaya
Ridge. Sex reversal of the grass shrimp, which is a proterandrous hermaphrodite, had a marked inﬂuence on the
concentrations of such metals as Fe, Cu, Cd, and Mn. The levels of Cd in the hepatopancreas of grass shrimp
exceeded maximum permissible concentrations for seafood at all the stations studied. The main factors deter-
mining the metal levels in
from the investigated locations are discussed.
: Heavy metals, grass shrimp,
, sex, upwellings, Kuril Islands