Mammalian Genome 12, 253–255 (2001). DOI: 10.1007/s003350010260 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2001 Construction of a rabbit bacterial artificial chromosome (BAC) library: application to the mapping of the major histocompatibility complex to position 12q1.1 1 1 2 1 1 1 Claire Rogel-Gaillard, Franc ¸ois Piumi, Alain Billault, Noelle Bourgeaux, Jean-Claude Save, Ce ´line Urien, 3 1 Je ´rome Salmon, Patrick Chardon Laboratoire INRA CEA de Radiobiologie et d’Etude du Ge ´nome, De ´partement INRA de Ge ´ne ´tique Animale, F-78350 Jouy-en-Josas, France Fondation Jean Dausset, Centre d’Etude du Polymorphisme Humain, F-75010 Paris, France INSERM U190, Institut Pasteur, 25-28 rue du Docteur Roux, F-75015 Paris, France Received: 11 July 2000 / Accepted: 16 October 2000 In the past five years, large DNA fragment libraries in Bacterial Artificial Chromosomes (BAC) (Shizuya et al. 1992) have permit- ted remarkable advances in the analysis of major domestic species of agronomic interest. Thus, BAC libraries representing from 3 to 10 haploid genome coverage are now available for a number of domestic animals. The success of the BAC libraries is mainly owing to the DNA fidelity of the genomic inserts and its low level of chimerism. In addition, BAC clones are amenable
Mammalian Genome – Springer Journals
Published: Mar 1, 2001
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