Construction of a phosphate transporter gene expression vector and its usage for tobacco transformation

Construction of a phosphate transporter gene expression vector and its usage for tobacco... A plant expression vector was constructed by inserting the phosphate transporter gene, LePT1, which was cloned from the tomato genome, into pCAMBIA2300. An agrobacterium-mediated system was used to transform tobacco and acquire transgenic plants. Analyses of the transgenic plants by PCR and RT-PCR indicated that the exogenous gene was integrated into and expressed by transgenic plants. The growth characteristics of T1 generation transgenic plants were examined, and the phosphate content of transgenic plants in a low-phosphate environment was found to be significantly higher than in wild-type plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Construction of a phosphate transporter gene expression vector and its usage for tobacco transformation

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Publisher
Springer Journals
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443713020210
Publisher site
See Article on Publisher Site

Abstract

A plant expression vector was constructed by inserting the phosphate transporter gene, LePT1, which was cloned from the tomato genome, into pCAMBIA2300. An agrobacterium-mediated system was used to transform tobacco and acquire transgenic plants. Analyses of the transgenic plants by PCR and RT-PCR indicated that the exogenous gene was integrated into and expressed by transgenic plants. The growth characteristics of T1 generation transgenic plants were examined, and the phosphate content of transgenic plants in a low-phosphate environment was found to be significantly higher than in wild-type plants.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Feb 17, 2013

References

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