Mammalian Genome 10, 1108–1111 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 Construction and characterization of an ovine bacterial artificial chromosome library 1 2 2 3 1 Clare A. Gill, Scott K. Davis, Jeremy F. Taylor, Noelle E. Cockett, Cynthia D.K. Bottema Department of Animal Science, Waite Campus, University of Adelaide, Glen Osmond, South Australia, 5064 Australia Department of Animal Science, Texas A&M University, College Station, Texas 77843-2471, USA Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, Utah 84322-4815, USA Received: 8 September 1998 / Accepted: 15 June 1999 Identification of regions of the genome that contain genes for bulk of the repetitive centromeric DNA that is resistant to diges- economically important traits in livestock species and isolation of tion with HindIII and remained in the compression band when the those genes so that they can be utilized in breeding programs fragments were separated by CHEF gel electrophoresis. requires high-density genome maps. Large-insert clones such as The partial digests with 0.75 U and 1 U HindIII were pooled in yeast artificial chromosomes (YAC), P1-derived artificial chromo- a single lane for size selection by gel electrophoresis. The CHEF somes (PAC), and bacterial artificial chromosomes (BAC)
Mammalian Genome – Springer Journals
Published: Nov 1, 1999
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