Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from pigs in China

Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from... Encephalomyocarditis virus (EMCV) infects animals of various species and causes a variety of clinical symptoms. In this study, an infectious full-length cDNA clone was constructed, and the characteristics of the rescued virus were investigated in vitro and in vivo . Our data demonstrated that the growth kinetics in vitro and plaque morphology of the rescued EMCV rNJ08 strain were similar to those of the parental strain. Although rNJ08 infected BALB/c mice, none of the mice died during the observation period of 14 days post-inoculation. The availability of the infectious cDNA clone provides a genetic platform for studying gene function and for the rational design of vaccines. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from pigs in China

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2290-1
Publisher site
See Article on Publisher Site

Abstract

Encephalomyocarditis virus (EMCV) infects animals of various species and causes a variety of clinical symptoms. In this study, an infectious full-length cDNA clone was constructed, and the characteristics of the rescued virus were investigated in vitro and in vivo . Our data demonstrated that the growth kinetics in vitro and plaque morphology of the rescued EMCV rNJ08 strain were similar to those of the parental strain. Although rNJ08 infected BALB/c mice, none of the mice died during the observation period of 14 days post-inoculation. The availability of the infectious cDNA clone provides a genetic platform for studying gene function and for the rational design of vaccines.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2015

References

  • Encephalomyocarditis in Korea: serological survey in pigs and phylogenetic analysis of two historical isolates
    An, DJ; Jeong, W; Jeoung, HY; Yoon, SH; Kim, HJ; Choi, CU; Park, BK
  • Protective efficacy of adenovirus-mediated small interfering RNAs against encephalomyocarditis virus challenge in mice
    Bai, J; Jiang, K; Zhang, L; Wang, X; Li, Y; Jiang, P
  • Genetic variability of encephalomyocarditis virus (EMCV) isolates
    Denis, P; Liebig, HD; Nowotny, N; Billinis, C; Papadopoulos, O; O’Hara, RS; Knowles, NJ; Koenen, F
  • Isolation, molecular characterization, and phylogenetic analysis of encephalomyocarditis virus from South China tigers in China
    Liu, H; Yan, Q; Zhao, B; Luo, J; Wang, C; Du, Y; Yan, J; He, H
  • Experimental encephalomyocarditis virus infection in calves
    Spradbrow, PB; Watt, DA; Chung, VS

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