Construction and characterization of a large insert porcine YAC library

Construction and characterization of a large insert porcine YAC library Mammalian Genome 8, 50-51 (1997). .enome 9 Springer-Verlag New York Inc. 1997 Construction and characterization of a large insert porcine YAC library* L.J. Alexander, 1 T.P.L. Smith, 1 C.W. Beattie, l M.F. Broom z 1U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, PO Box 166, State Spur 18D, Clay Center, Nebraska 68933, USA 2AgReseareh Molecular Biology Unit, Department of Biochemistry and Centre for Gene Research, University of Otago, 676 Cumberland St., PO Box 56, Dunedin, New Zealand Received: 20 May 1996 / Accepted: 22 August 1996 with a median size of 480 kb. Thus, the predicted level of coverage The recent construction of genetic linkage maps of the porcine genome (Rohrer et al. 1994, 1996; Ellegren et al. 1994; Archibald is theoretically 5.3 (median) to 6.5 (mean) genome equivalents, et al. 1995) allows the assignment of loci affecting heritable traits assuming a 3 billion bp genome. of economic importance (ETLs; Lander and Botstein 1989) to The number of genome equivalents in the library was more specific chromosomal segments. Markers can thus be identified rigorously defined by subjecting the 23 primary DNA pools to that may be useful in marker-assisted selection (MAS) to Mammalian Genome Springer Journals

Construction and characterization of a large insert porcine YAC library

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Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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