Conserved genomes of ΦKMV-like bacteriophages (T7 supergroup) active on Pseudomonas aeruginosa

Conserved genomes of ΦKMV-like bacteriophages (T7 supergroup) active on Pseudomonas aeruginosa The T7-like ΦKMV bacteriophage active on Pseudomonas aeruginosa was previously isolated by us and shown to have DNA resistant to many endonucleases. A loss of sensitive sites might be a consequence of a long ΦKMV evolution on different hosts. To elucidate, whether this trait is shared by other similar phages, several new ΦKMV-like phages were isolated from different sources and compared. All studied ΦKMV-like phages formed three groups, insignificantly differing in the number and localization of endonuclease-sensitive DNA sites. This confirms that the present-day phages of this species have highly conserved genomes. Mutational “restoration” of the lost sites may be restricted by a lethal effect. The ΦKMV-like phages were shown for the first time to increase the rate of in vitro accumulation of giant KZ-like phages of P. aeruginosa. This effect is characteristic only of ΦKMV-like phages. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Conserved genomes of ΦKMV-like bacteriophages (T7 supergroup) active on Pseudomonas aeruginosa

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by Pleiades Publishing, Inc.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795406010030
Publisher site
See Article on Publisher Site

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