1063-0740/01/2703- $25.00 © 2001
Russian Journal of Marine Biology, Vol. 27, No. 3, 2001, pp. 184–187.
Original Russian Text Copyright © 2001 by Biologiya Morya, Kartavtsev, Malinovskaya, Khristoforova, Amachaeva, Nikiforov.
In recent decades, monitoring of the quality of the
marine environment and the evaluation of the degree of
pollution, including heavy metal pollution, have
remained necessary. From the biological standpoint, it
is of particular interest to compare heavy metal concen-
trations in organisms that inhabit protected territories
or waters (various types of nature reserves) and those of
inhabitants of industrial and urbanized zones.
The predatory gastropod
idae) is common in the northwestern part of the Sea of
Japan, where it is found on hard substrates at depths of
2 or 3 m. Mollusks of this species were used as indica-
tor organisms in comparing the pollution levels in two
areas of Peter the Great Bay, the eastern coast of Amur-
skii Bay and the western coast of Vostok Bay. It is
known that in the ﬁrst area, the sea water is heavily pol-
luted due to the high urbanization of the coastal region
(the cities of Vladivostok and Artem and their suburbs),
while the second area, which, for the greater part, is a
nature reserve, is only locally exposed to industrial pol-
lution. By analyzing the variability of metal concentra-
tions in mollusks from such contrasting habitats, it is
possible to achieve two objectives: ﬁrst, to ﬁnd differ-
ences between populations due to environmental pollu-
tion, and, second, if signiﬁcant changes related to habitat
conditions are found, to understand their value as an
adaptive response to environmental changes.
This study focuses on the analysis of intra- and
interpopulation variations in heavy metal concentra-
tions in soft tissues of the gastropod
under contrasting environmental conditions.
MATERIALS AND METHODS
Mollusks were collected in 1997 in Amurskii Bay
on the northern (7 August), southern (3 September),
and western (31 August) coasts of Skrebtsov Islet
200 m), located at 300 m away from the eastern
coast: samples AM1–AM3 consist of 35, 35, and 31
), respectively (in all, 101 individuals). In
Vostok Bay, mollusks (67 individuals) were also sam-
pled in 1997 in Srednyaya Bay (10 October, sample
= 35) and at Cape Pashinnikov near the Vostok
Biological Station (14 October, sample VS5,
= 32). A
total of 168 mollusks were surveyed. The sampling
depth in both bays was 0.5 to 1.5 m.
Soft tissues were dried at 85
C and mineralized with
concentrated nitric acid. The mineralizates were diluted
with a 2% solution of HNO
and were analyzed for the
contents of six elements (Cu, Zn, Cd, Pb, Fe, and Mn)
using atomic absorption spectrophotometry. Metal con-
centrations are given in micrograms per gram of dry tis-
sue (except foot tissue) of individual mollusk.
Concentrations of Heavy Metals in Soft Tissues
of the Gastropod
in the Vicinity
of Vladivostok and in the Vostok Bay Reserve
Yu. F. Kartavtsev
, T. M. Malinovskaya
, N. K. Khristoforova
E. Yu. Amachaeva
, and S. M. Nikiforov
Institute of Marine Biology, Far East Division, Russian Academy of Sciences, Vladivostok, 690041 Russia
Institute of Geography, Far East Division, Russian Academy of Sciences, Vladivostok, 690041 Russia
Received October 23, 2000
—A survey was made of 168 specimens of the gastropod mollusk
from 5 samples
collected in Amurskii Bay and Vostok Bay (Peter the Great Bay and the Sea of Japan). The variability of Cu,
Zn, Cd, Pb, Fe, and Mn contents in gastropod soft tissues was analyzed. The statistic concentrations of the six
metals differed signiﬁcantly among the samples; they were sometimes taken at a distance of a mere several tens
of meters apart.
collected near Vladivostok generally had higher concentrations of heavy metals;
however, the amount of Zn in the specimens of one sample from the Vostok Bay Reserve was 400
is two times greater than that of mollusks from the urbanized zone. The canonical and discriminant analyses
and the analysis of the variance indicate that the variation in heavy metal concentration within groups of mol-
lusks (within bays) is comparable in magnitude with the variation among the groups.
heavy metals, mollusk soft tissues.