Computational identification of miRNA genes and their targets in mulberry

Computational identification of miRNA genes and their targets in mulberry MicroRNAs (miRNA) are a class of tiny non protein coding and regulatory RNA molecules about 18 to 26 nt in length. miRNA regulate gene expression via the degradation or translational inhibition of their target mRNAs. Nucleotide sequences of miRNAs are highly conserved among various organisms; this forms the key feature behind the identification of miRNAs in plant species by homology alignment. So far, little is known about miRNA in mulberry (Morus alba L.) species. In our study, a computational method was used for detection of mulberry miRNAs. A total of six miRNAs were identified. The six miRNAs may regulate twenty-two potential targets, which are predicted to encode transcription factors that regulate plant development, signaling, and metabolism. To validate the prediction of miRNAs in mulberry, a RT-PCR experimental method was performed and five of these miRNAs were found to be expressed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Computational identification of miRNA genes and their targets in mulberry

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Publisher
Pleiades Publishing
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714040104
Publisher site
See Article on Publisher Site

Abstract

MicroRNAs (miRNA) are a class of tiny non protein coding and regulatory RNA molecules about 18 to 26 nt in length. miRNA regulate gene expression via the degradation or translational inhibition of their target mRNAs. Nucleotide sequences of miRNAs are highly conserved among various organisms; this forms the key feature behind the identification of miRNAs in plant species by homology alignment. So far, little is known about miRNA in mulberry (Morus alba L.) species. In our study, a computational method was used for detection of mulberry miRNAs. A total of six miRNAs were identified. The six miRNAs may regulate twenty-two potential targets, which are predicted to encode transcription factors that regulate plant development, signaling, and metabolism. To validate the prediction of miRNAs in mulberry, a RT-PCR experimental method was performed and five of these miRNAs were found to be expressed.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jun 24, 2014

References

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