Comprehensive transcriptome analysis of Lactuca indica, a traditional medicinal wild plant

Comprehensive transcriptome analysis of Lactuca indica, a traditional medicinal wild plant Lactuca indica Linn. (Indian lettuce) is an undomesticated, traditional medicinal plant belonging to the Compositae family. Here, we performed transcriptome assembly and functional annotation of this medicinal plant, along with secondary metabolite analysis. Sesquiterpene lactones, the most abundant secondary metabolites in Lactuca species, are mainly responsible for the medicinal properties of Lactuca. We therefore measured the levels of lactucin, a primary sesquiterpene lactone, in 61 accessions of L. indica, which varied from 1.9 μg/g to 98.7 μg/g. De novo transcriptome assembly yielded 73,300 unigenes from 127 million reads based on 12.9 Gb of data. In total, 28,970 and 34,519 unigenes were annotated using the Swiss-Prot and TAIR10 databases, respectively. The most highly enriched Gene Ontology term for these unigenes was metabolic processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified 97 significant pathways; the top two pathways were metabolic pathways and biosynthesis of secondary metabolites. Among the 9743 unigenes mapped to 97 significant KEGG pathways, 52 complete unigenes encoding 23 key enzymes in the terpenoid backbone pathway were identified. We identified 1685 transcription factor genes belonging to 53 families and 8830 simple sequence repeat (SSR) loci based on the transcripts. The high-quality transcriptomic and metabolic data on this indigenous herb obtained in this study represent a valuable genetic resource for breeding an L. indica cultivar with excellent pharmaceutical benefits. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Breeding Springer Journals

Comprehensive transcriptome analysis of Lactuca indica, a traditional medicinal wild plant

Loading next page...
 
/lp/springer_journal/comprehensive-transcriptome-analysis-of-lactuca-indica-a-traditional-GSHc7piBNG
Publisher
Springer Netherlands
Copyright
Copyright © 2017 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Sciences; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Biotechnology
ISSN
1380-3743
eISSN
1572-9788
D.O.I.
10.1007/s11032-017-0711-z
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial