2018 Springer Science+Business Media, LLC
Chemistry of Natural Compounds, Vol. 54, No. 3, May, 2018
COMPOSITION AND ANTIOXIDANT, ANTICHOLINESTERASE,
AND ANTIBACTERIAL ACTIVITIES OF THE ESSENTIAL OIL
OF Salvia buchananii FROM ALGERIA
Khadidja Aya Beladjila,
and Zahia Kabouche
The genus Salvia (Lamiaceae) is represented by more than 900 species distributed worldwide . In Algeria, there
are 23 species from which S. jaminiana, S. verbenaca subsp., S. clandestina, S. barrilieri, and S. argentea subsp. aurasiaca
were the subject of our studies [2–7]. We present here the first report on the essential oil composition, the antioxidant,
anticholinesterase, and the antibacterial activities of the species Salvia buchananii Hedge which has been previously investigated
for its phytochemicals [8, 9].
Extraction. The hydrodistillation, in a Clenvenger-type apparatus, of fresh aerial parts (200 g) of Salvia buchananii,
collected at Constantine in August 2015 (Voucher number: LOST Sb08.16), yielded 0.9% of a yellow fragrant essential oil.
GC and GC-MS Analyses. GC analysis was performed on a Hewlett Packard 6890 gas chromatograph equipped
with an FID and HP-5MS capillary column. The GC/MS was performed using a Hewlett Packard 6890 gas chromatograph
coupled with a Hewlett Packard HP (Agilent technologies) MSD 5973 selective detector, using an HP-INNOWax column
(30 m u 0.25 mm, film thickness 0.25 Pm) .
Identification of Components. Essential oil components were identified based on their retention indices (determined
with reference to a homologous series of normal alkanes) and by comparison of their mass spectral fragmentation patterns
with those reported in the literature [11, 12] and with authentic compounds, especially for major components.
Thirteen components, representing 95.9% of the total oil content, were identified with 1,8-cineole (23.3%),
(19.8%), camphene (16.5%),
-pinene (11.5%), and bornyl acetate (5.5%) as the major components (Table 1). This is the first
report on the essential oil of S. buchananii. In previous studies of the essential oils of the genus, 1,8-cineole was found to be
the major component of S. potentillifolia (19.3%) , S. fructicosa (47.5%) , S. apiana (71.7%) , and S. macrochlamys
(27.0%)  essential oils. In agreement with the literature, the present oil contains high amounts of bicyclic monoterpenes.
-pinene have been found as major components of the essential oil of S. potentillifolia (29.3%, 14.8%) .
It is noteworthy that
-caryophyllene (16.0%, 24.8%, 19.0%) was mainly present in the essential oils of S. verticillata,
S. aethiopis, and S. nemorosa . Moreover, camphene and camphor (8.5%, 43.8%) were mainly identified in the essential
oils of S. officinalis from Albania .
Antioxidant Activity. The antioxidant activity was assessed using the
-carotene-linoleic acid test .
The essential oil showed good antioxidant activity, with 50.48% inhibition at 4 mg/mL, 50.27% at 2 mg/mL and
42.96% at 1 mg/mL.
1) Universite des Freres Mentouri Constantine, Departement de Chimie, Laboratoire dcObtention de Substances
Therapeutiques (LOST), 25000, Constantine, Algeria, fax: +21331811100, e-mail: firstname.lastname@example.org; 2) Department of
Chemistry, Faculty of Science, The University of Jordan, Amman, Jordan; 3) CHU Benbadis–Constantine, Service de
Bacteriologie, 25000, Constantine, Algeria; 4) Department of Physics and Basic Sciences, Faculty of Engineering Technology
Al-Balqa Applied University, Amman, Jordan; 5) Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques
(C.R.A.P.C), Alger, Algerie. Published in Khimiya Prirodnykh Soedinenii, No. 3, May–June, 2018, pp. 494–495.
Original article submitted October 4, 2016.