Arch Virol (2005) 150: 403–405 DOI 10.1007/s00705-004-0423-7 Complete sequence of the RNA2 of an isolate of White clover cryptic virus 1, type species of the genus Alphacryptovirus Annotated Sequence Record 1 2 G. Boccardo and T. Candresse Istituto di Virologia Vegetale, CNR, Torino, Italy Equipe de Virologie, INRA et Université Bordeaux 2, Villenave d’Ornon, France Received August 16, 2004; accepted August 16, 2004 Published online October 21, 2004 Springer-Verlag 2004 Introduction White clover cryptic virus 1 (WCCV1) is the type species of the genus Alphacryp- tovirus, family Partitiviridae but no sequence data have been available for either of its two genomic dsRNAs or the proteins they encode. A companion paper  now reports on the complete sequence of RNA1 of the virus, and here we report on the complete sequence of RNA2. Provenance of the virus material The WCCV1 isolate used was obtained from naturally infected white clover seedlings as described previously [2, 3]. Viral cDNAs were prepared from puriﬁed WCCV1 dsRNAs extracted from puriﬁed viral particles  using the technique of Gubler and Hoffman  following oligo-dT priming. The 5 -terminal sequence was conﬁrmed by primer extension on the puriﬁed viral dsRNAs. The sequence obtained has been
Archives of Virology – Springer Journals
Published: Feb 1, 2005
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