Complete sequence of the RNA2 of an isolate of White clover cryptic virus 1 , type species of the genus Alphacryptovirus

Complete sequence of the RNA2 of an isolate of White clover cryptic virus 1 , type species of the... Arch Virol (2005) 150: 403–405 DOI 10.1007/s00705-004-0423-7 Complete sequence of the RNA2 of an isolate of White clover cryptic virus 1, type species of the genus Alphacryptovirus Annotated Sequence Record 1 2 G. Boccardo and T. Candresse Istituto di Virologia Vegetale, CNR, Torino, Italy Equipe de Virologie, INRA et Université Bordeaux 2, Villenave d’Ornon, France Received August 16, 2004; accepted August 16, 2004 Published online October 21, 2004  Springer-Verlag 2004 Introduction White clover cryptic virus 1 (WCCV1) is the type species of the genus Alphacryp- tovirus, family Partitiviridae but no sequence data have been available for either of its two genomic dsRNAs or the proteins they encode. A companion paper [1] now reports on the complete sequence of RNA1 of the virus, and here we report on the complete sequence of RNA2. Provenance of the virus material The WCCV1 isolate used was obtained from naturally infected white clover seedlings as described previously [2, 3]. Viral cDNAs were prepared from purified WCCV1 dsRNAs extracted from purified viral particles [2] using the technique of Gubler and Hoffman [5] following oligo-dT priming. The 5 -terminal sequence was confirmed by primer extension on the purified viral dsRNAs. The sequence obtained has been http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Complete sequence of the RNA2 of an isolate of White clover cryptic virus 1 , type species of the genus Alphacryptovirus

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Publisher
Springer Journals
Copyright
Copyright © 2005 by Springer-Verlag/Wien
Subject
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-004-0423-7
Publisher site
See Article on Publisher Site

Abstract

Arch Virol (2005) 150: 403–405 DOI 10.1007/s00705-004-0423-7 Complete sequence of the RNA2 of an isolate of White clover cryptic virus 1, type species of the genus Alphacryptovirus Annotated Sequence Record 1 2 G. Boccardo and T. Candresse Istituto di Virologia Vegetale, CNR, Torino, Italy Equipe de Virologie, INRA et Université Bordeaux 2, Villenave d’Ornon, France Received August 16, 2004; accepted August 16, 2004 Published online October 21, 2004  Springer-Verlag 2004 Introduction White clover cryptic virus 1 (WCCV1) is the type species of the genus Alphacryp- tovirus, family Partitiviridae but no sequence data have been available for either of its two genomic dsRNAs or the proteins they encode. A companion paper [1] now reports on the complete sequence of RNA1 of the virus, and here we report on the complete sequence of RNA2. Provenance of the virus material The WCCV1 isolate used was obtained from naturally infected white clover seedlings as described previously [2, 3]. Viral cDNAs were prepared from purified WCCV1 dsRNAs extracted from purified viral particles [2] using the technique of Gubler and Hoffman [5] following oligo-dT priming. The 5 -terminal sequence was confirmed by primer extension on the purified viral dsRNAs. The sequence obtained has been

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2005

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