Arch Virol (2007) 152: 839–841 DOI 10.1007/s00705-006-0886-9 Printed in The Netherlands Annotated Sequence Record Complete sequence of the RNA1 of a European isolate of tomato chlorosis virus G. Lozano, E. Moriones, and J. Navas-Castillo Estaci o on Experimental La Mayora, Consejo Superior de Investigaciones Cient´ ıﬁcas, Algarrobo-Costa, Ma ´laga, Spain Received September 6, 2006; accepted November 11, 2006; published online December 14, 2006 # Springer-Verlag 2006 Introduction Provenance of the virus material Tomato chlorosis virus (ToCV, genus Crinivirus, Isolate AT80=99 of ToCV was obtained during family Closteroviridae) is the causal agent of a 1999 from a naturally infected tomato plant from ‘‘yellow leaf disorder’’ of tomato (Solanum lyco- Malaga (southern Spain) and was maintained in persicum L.) that has been present in Florida at tomato cv. Moneymaker by periodic transmission least since 1989 . Nowadays, the presence of using the whiteﬂy Bemisia tabaci (Q biotype) as ToCV has been conﬁrmed in many countries of the vector. Total RNA or double-stranded-RNA-enriched Caribbean, the Far East, southern Africa, and the preparations from infected tomato plants were used in northern and southern sides of the Mediterranean RT-PCR reactions to clone and sequence the RNA1 Basin. In Spain, ToCV has been present in
Archives of Virology – Springer Journals
Published: Apr 1, 2007
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