Complete sequence of a cryptic virus from hemp ( Cannabis sativa )

Complete sequence of a cryptic virus from hemp ( Cannabis sativa ) Hemp ( Cannabis sativa ) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous particles, spherical particles with a diameter of around 34 nm were found. RNA from virus preparations was purified, and cDNA was prepared and cloned. Sequence information was used to search databases, and the greatest similarity was found with Primula malacoides virus 1, a putative new member of the genus Partitivirus . The full sequences of RNA 1 and RNA 2 of this new hemp cryptic virus were obtained. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Complete sequence of a cryptic virus from hemp ( Cannabis sativa )

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Publisher
Springer Journals
Copyright
Copyright © 2012 by Springer-Verlag
Subject
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-011-1168-8
Publisher site
See Article on Publisher Site

Abstract

Hemp ( Cannabis sativa ) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous particles, spherical particles with a diameter of around 34 nm were found. RNA from virus preparations was purified, and cDNA was prepared and cloned. Sequence information was used to search databases, and the greatest similarity was found with Primula malacoides virus 1, a putative new member of the genus Partitivirus . The full sequences of RNA 1 and RNA 2 of this new hemp cryptic virus were obtained.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2012

References

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